小儿止咳颗粒对哮喘大鼠气道高反应性和炎症的抑制作用

目的:探讨小儿止咳颗粒对哮喘大鼠气道高反应性的改善作用及其机制。方法:大鼠第0,8天sc及ip给予1mL含卵蛋白(OVA)100 mg的氢氧化铝凝胶和1 mL含卡介苗3 mg的生理盐水致敏,第15天开始以OVA攻击制备哮喘模型,同时ig给予小儿止咳颗粒高、中、低剂量(15.7,7.85,3.925 g·kg-1)。第22天测定大鼠气道阻力、血清中高迁移率族蛋白B-1(HMGB-1),肺匀浆中免疫球蛋白E(IgE),白介素-4(IL-4),白介素-13(IL-13)的含量。结果:小儿止咳颗粒高、中、低剂量均可明显抑制引喘后20,30,40,60 s大鼠气道所需正压力的上升(与模型组相比较P<0.01);小儿止咳颗粒各剂量组可明显抑制血清HMGB-1及肺匀浆IgE含量的增高(P<0.05,P<0.01);小儿止咳颗粒高、中剂量可以明显抑制肺匀浆IL-13,IL-4含量的增高(P<0.01)。结论:小儿止咳颗粒可以通过抑制炎症介质、细胞因子含量的增高降低哮喘大鼠的气道高反应性,从而治疗小儿上呼吸道感染后咳嗽。

Inhibitory Effects of Xiaoer Zhike Granule on Ovalbumin-inducedLung Inflammation and Airway Hyperresponsiveness in Asthmatic Rats

Objective: To investigate the effects of Xiaoer Zhike Granule on airway hyperresponsiveness and inflammation in asthmatic rats. Method: The rat model was performed by intraperitoneal injection of 1mL A1(OH)₃ gel containing OVA 100 mg and 1mL NS containing BCG vaccine 3 mg. The rats were constructed by immunization of intraperitoneal injection on the 0 day and the 7^th day,and the experiment was performed on the 15^th day, while giving Xiaoer Zhike Granule high, medium and low doses (15.7, 7.85, 3.925 g ·kg^-1 respectively). On the 22^th day airway resistance rats was tested. The content of serum HMGB-1 and IgE, IL-4, IL-13 in lung homogenate were determined. Result: Xiaoer Zhike Granule at high, medium and low doses could inhibit airway pressure in rats ( P <0.01), it could inhibit the increasing in serum HMGB-1 and lung homogenate IgE content ( P <0.05, 0.01). The high and medium doses could inhibit the content increasing of lung homogenate IL-13, IL-4 ( P <0.01). Conclusion: Xiaoer Zhike Granule can lower airway responsiveness in asthma rats by inhibiting inflammation, cytokine content, so that it can treat pediatric coughing after upper respiratory tract infection.