益气活血方药抑制大鼠HSC体外增殖作用的实验研究

目的　研究益气活血方药抑制大鼠肝星状细胞 (HSC)体外增殖的作用机理和有效部位群。方法　用脂多糖 (lipopolysccharide ,LPS)刺激体外培养的HSC增殖 ,同时加入不同时相的含药血清Scl、Sc2或正常鼠血清 (Sn)或该方药不同提取物 ,分别培养 2 4、4 8、72h ,MTT法测定细胞增殖率 ,流式细胞仪测定HSC凋亡率。结果　在培养各时相Sc1和Sc2对LPS刺激的HSC增殖都有明显的抑制作用 (P <0 0 5或P <0 0 1)。流式细胞仪测定HSC凋亡的结果显示Sc2明显促进了HSC的凋亡 (P <0 0 1)。该方药不同提取物以不同的模式抑制体外培养的大鼠HSC增殖。结论　益气活血方药含药血清具有抑制体外培养大鼠HSC增殖作用 ,并促进其凋亡 ;益气活血方药有不同的有效部位群 ,并以不同的模式发挥作用

Experimental Study on the Inhibitive Effects of Yiqihuoxue Formula on the Proliferation of Rat Hepatic Stellate Cells in Vitro

Objective To study the mechanism of the effects of Yiqihuoxue formula (YF) in inhibiting the proliferation of hepatic stellate cells (HSC) in vitro, and to investigate the different active extracts of the formula. Methods The proliferation of HSC was stimulated by lipopolysaccharide (LPS) in vitro, and then, the YF-medicated rat serum at different time phases (Sc1 and Sc2), or normal rat serum, or different YF extracts were added, and cultivated for 24 hs, 48 hs and 72 hs, respectively. MTT assay was used to detect HSC proliferative rate and the flow cytometry (FCM) was used to detect HSC apoptotic rate. Results Both Sc1 and Sc2 could significantly inhibited the proliferation of HSC induced by LPS at different time phases (P<0.05, P<0.01). The results from FCM showed that Sc2 remarkably promoted the apoptosis of HSC (P<0.01). It was also found that different YF extracts could inhibit the proliferation of rat HSC cultured in vitro on different modes. Conclusion YF-medicated serum can restrain the proliferation of rat HSC in vitro and promote HSC apoptosis as well. YF contains different effective fractions which exert their effects on different modes.