马传染性贫血病毒驴白细胞弱毒疫苗gag基因的序列测定及分析

以马传染性贫血病毒（ＥＬＡＶ）驴白细胞弱毒疫苗株（ＤＬＡ）病毒基因组ＲＮＡ为材料，用ＲＴ－ＰＣＲ方法扩增出ＥＩＡＶ ｇａｇ基因，以平端针其克隆到质粒载体ｐＵＣ１９中，由于疫苗不是克隆株，因此通过５次单独克隆与测序，推导出ＥＩＡＶ－ＤＬＡ ｇａｇ基因的优势序列。ｇａｇ基因全长１４５８个碱基，编码一４８６个氨基酸残基的前体蛋白。与美国ＥＩＡＶ Ｗｙｏｍｉｎｇ１３６９株比较，核苷酸同源性为８０％，氨基酸

Sequencing and Analysis of gag Gene of Donkey Leukocyte Attenuated Equine Infectious Anemia Virus

In order to study the attenuated mechanism of donkey leukocyte attenuated (DLA) equine infectious anemia virus (EIAV), the genome RNA of DLA EIAV was used to amplify the gag gene by RT PCR, and the PCR product was cloned to the plasmid pUC19. The cloned gag gene originated from DLA EIAV RNA was further cloned and sequenced for another five times. The resultant DLA EIAV gag gene includes 1,458 bp, which encodes a 486 amino acid precursor protein. The DLA EIAV gag shared the homology of 80% in nucleotide and 84.47% in amino acid with the EIAV Wyoming 1369 strain. Our results demonstrated that there are highly conserved structures in the major homology region (MHR) of capsid protein P26, two CCHC type zinc fingers of nucleocapsid P11 and the YXXL motif in the P9 between DLA EIAV and Wyoming 1369.