| LC-MS/MS法测定保健食品中左卡尼汀的含量 |
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| 引用本文： | 王莎,郭彦丽,周提军,城田修.LC-MS/MS法测定保健食品中左卡尼汀的含量[J].食品安全质量检测技术,2012,3(4):309-312. |
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| 作者姓名： | 王莎 郭彦丽 周提军 城田修 |
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| 作者单位： | 资生堂(中国)投资有限公司先端科学事业推进部应用技术中心,资生堂(中国)投资有限公司先端科学事业推进部应用技术中心,资生堂(中国)投资有限公司先端科学事业推进部应用技术中心,资生堂(中国)投资有限公司先端科学事业推进部应用技术中心 |
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| 摘 要： | 目的 建立保健品中左卡尼汀的LC-MS/MS的测定方法。方法 待测样品经超声提取、稀释后, 分别采用CAPCELL PAK CR色谱柱和PC HILIC色谱柱进行分离, 最后用LC-MS/MS进行检测。 结果 使用CAPCELL PAK CR法检测左卡尼汀能够得到更高的灵敏度, 线性范围为0~200 ng/mL, 相关系数为r=0.9995。结论 该方法灵敏度高、准确、重现性好, 可以用于一些保健食品中左卡尼汀的含量检测。
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| 关 键 词： | 左卡尼汀 LC-MS / MS 保健食品 |
| 收稿时间： | 2012/7/10 0:00:00 |
| 修稿时间： | 8/7/2012 12:00:00 AM |
Determination of levocarnitine in health care food by LC-MS/MS |
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| WANG Sh,GUO Yan-Li,ZHOU Ti-Jun and CHENG Tian-Xiu.Determination of levocarnitine in health care food by LC-MS/MS[J].Food Safety and Quality Detection Technology,2012,3(4):309-312. |
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| Authors: | WANG Sh GUO Yan-Li ZHOU Ti-Jun and CHENG Tian-Xiu |
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| Affiliation: | Advanced Science and Business Promotion Division Applied Technology Center of Shiseido (China) Investment Co., Ltd.,Advanced Science and Business Promotion Division Applied Technology Center of Shiseido (China) Investment Co., Ltd.,Advanced Science and Business Promotion Division Applied Technology Center of Shiseido (China) Investment Co., Ltd.,Advanced Science and Business Promotion Division Applied Technology Center of Shiseido (China) Investment Co., Ltd. |
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| Abstract: | Objective To establish a method for the determination of levocarnitine in health care food by LC-MS/MS. Methods After an ultrasonic extraction and dilution, the samples were separated by CAPCELL PAK CR and PC HILIC column, respectively. Finally the target compound was detected by LC-MS/MS. Results Compared with the PC HILIC column, using CAPCELL PAK CR column could get a higher sensitivity result. The method showed a good linearity over the range of 0~200.0 ng/mL for levocarnitine with r=0.9995. Conclusion This method has a good sensitivity, accuracy and reproducibility and it is suitable for the detection of levocarnitine in health care food. |
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| Keywords: | levocarnitine LC-MS/MS health care food |
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