mTOR信号通路相关蛋白在胰腺癌中的表达及其临床意义

目的 探讨mTOR信号通路相关蛋白在胰腺癌中的表达及其临床意义。方法 采用免疫组织化学技术测定并比较mTOR信号通路相关蛋白Akt、mTOR及P70S6K在胰腺癌和非胰腺癌组织中的磷酸化水平，应用Spearman相关分析探讨mTOR信号通路相关蛋白磷酸化水平与胰腺癌患者年龄、性别、肿瘤大小、组织学分级、淋巴结转移及肿瘤分期的相关性。结果 p-Akt和p-mTOR蛋白阳性着色定位于细胞质内，而p-P70S6K蛋白阳性着色定位于细胞核。p-mTOR、p-Akt和p-P70S6K蛋白在胰腺癌组织中表达水平显著高于非胰腺癌组织（P均< 0.01）。Spearman 相关分析显示，p-Akt、p-mTOR和p-P70S6K蛋白表达与患者年龄、性别、肿瘤大小、组织学分级无关（P均>0.05），与淋巴结转移和分期有关（P均<0.05）。结论 mTOR信号通路与胰腺癌的发生发展相关，但mTOR信号通路相关蛋白磷酸化水平在胰腺癌临床分型及转归中的意义不大。

Expression and clinical significance of mTOR signaling pathway- associated proteins in pancreatic cancer

Objective To investigate the expression and clinical significance of mTOR signaling pathway- associated proteins in pancreatic cancer. Methods The phosphorylation levels of the mTOR signaling pathway- associated proteins, including Akt, mTOR and P70S6K, were detected and compared by the immunohistochemical technique in tissues of pancreatic cancer and non- pancreatic cancer. The correlations among the phosphorylation levels of the mTOR signaling pathway- associated proteins and the patients’age, gender, tumor size, histological grading, lymph node metastasis and tumor stage were evaluated by Spearman correlation analysis. Results The positive staining of p- Akt and p- mTOR protein was mainly present in the cytoplasm, while p- P70S6K was in the cell nucleus. The expression of p- mTOR, p- Akt and p- P70S6K were significantly higher in pancreatic cancer tissues than those in non- pancreatic cancer tissues （all P values 〈 0.01）. Spearman correlation analysis revealed the expression of p- Akt, p- mTOR and p- P70S6K had no associations with the patients’age, gender, tumor size and histological grading （all P values〉0.05）, while it correlated with lymph node metastasis and tumor staging （both P values〈0.05）. Conclusion mTOR signaling pathway correlateed with the development and progression of pancreatic cancer; however, the phosphorylation levels of the mTOR signaling pathwayassociated proteins had little significance in the clinical classification and prognosis of pancreatic cancer.