| 黑曲霉 G-1125生淀粉糖化酶基因的克隆与序列分析 |
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| 引用本文： | 孙海彦,黎娟华,刘恩世,易小平,杨景豪,尹一伊,彭明.黑曲霉 G-1125生淀粉糖化酶基因的克隆与序列分析[J].中国酿造,2015,34(2):51-54. |
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| 作者姓名： | 孙海彦 黎娟华 刘恩世 易小平 杨景豪 尹一伊 彭明 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所，海南海口571101 |
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| 基金项目： | 国家自然科学基金(31000029);国家高技术研究发展计划“863计划”(2012AA101204);海南省自然科学基金(ZDFD 20120765);海南省研究生创新科研课题(Hyb2011-4);海南省引进集成应用专项(YJJC2011004);海南省重大科技项目(ZDZX2013023-1);中国热带农业科学院中央级公益院所基本科研业务费专项(ITBB2015RC06) |
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| 摘 要： | 从黑曲霉Aspergillus niger G-1125克隆到了生淀粉糖化酶菌基因的DNA及cDNA序列。序列分析表明，该生淀粉糖化酶基因组DNA 序列编码区长2 172 bp，cDNA编码区长1 923 bp，该基因含有4个内含子，共编码640个氨基酸，前18个氨基酸为信号肽序列，该氨基酸序列中共含有2个潜在的糖基化位点，软件预测出该酶的分子质量约为68 ku，等电点为pH值4.07。
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| 关 键 词： | 黑曲霉 生淀粉糖化酶 克隆 序列分析 |
Cloning and sequence analysis of gene encoded raw-starch-digesting-glucoamylase from Aspergillus niger G-1125 |
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| SUN Haiyan;LI Juanhua;LIU Enshi;YI Xiaoping;YANG Jinghao;YIN Yiyi;PENG Ming.Cloning and sequence analysis of gene encoded raw-starch-digesting-glucoamylase from Aspergillus niger G-1125[J].China Brewing,2015,34(2):51-54. |
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| Authors: | SUN Haiyan;LI Juanhua;LIU Enshi;YI Xiaoping;YANG Jinghao;YIN Yiyi;PENG Ming |
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| Affiliation: | Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China |
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| Abstract: | DNA and cDNA of raw-starch-digesting-glucoamylase were cloned from Aspergillus niger G-1125. The sequence analysis revealed that the length of DNA and cDNA of this gene were 2 172 bp and 123 bp, respectively. The DNA sequence contained 4 introns and encoded a protein of 640 amino acids with a signal peptide of 18 amino acids and two potential glycosylation sites. The calculated molecular weight and isoelectric point of the enzyme were 68 ku and pH 4.07, respectively. |
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| Keywords: | Aspergillus niger raw-starch-digesting-glucoamylase cloning sequence analysis |
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