人类小细胞肺癌细胞转染野生型p53基因发生的凋亡

为了探索p5 3抑癌基因在临床应用中的潜在价值 ,以带有突变型p5 3基因的人类小细胞肺癌细胞株 (LTEP P)为研究对象 ,开发了一种新型的基因转染方法。将构建有野生型p5 3基因cDNA的质粒与阳离子脂质体和转铁蛋白结合成三元复合物 ,再用于LIEP P细胞的基因转染。通过显微观察、吖啶橙荧光染色、琼脂糖凝胶电泳和切口末端标记试验 ,证实细胞发生了凋亡。表明在体外实验中 ,该三元复合物可有效地转染肿瘤细胞 ,而野生型p5 3基因能够诱导肿瘤细胞发生凋亡。

Study on Apoptosis of Human Small cell Lung Carcinoma Cells Induced by Transfection of Wild type p53 Gene

In order to study on the potential effect of p53 tumor suppressor gene, a novel method for gene transfection was developed.The plasmid pCEP4-p53 containing the cDNA of wild type p53 gene was combined with cation liposome and transferrin,then transfected to LTEP-P cells. Cell apoptosis was proved by microscopy,acridine orange (AO) staining,agarose gel electrophoresis and nick end labeling test.The result showed that tumor cells were successfully transfected with the DNA-liposome-transferrin triple complex in vitro, and the apoptosis of tumor cells were induced by wild type p53 gene.