18个优质小麦品种(系)Glu-1、Glu-3和Gli-1位点的基因变异特点

为给小麦品质育种提供参考信息,选用我国18份有影响的优质面条和面包小麦品种或种质资源，通过分步法SDS-PAGE和改良A-PAGE分析了其Glu-1、Glu-3、Gli-1位点的基因变异特点。结果表明，由Glu-1住点控制的高分子量谷蛋白亚基共14种图谱，其中，Glu-A1位点上优质亚基1和2^＊分别占61．1％和11．1％，Glu—B1位点上优质亚基14＋15、7＋8、17＋18、13＋16分别占27．8％、27．8％、22．2％、5．6％．Glu_D1位点上优质亚基5＋10占55．6％。Glu—A1、Glu—B1、Glu-D1位点上均为优质亚基的品种（系）有陕451（1，7＋8，5＋10）、95鉴5104（1,14＋15，5＋10）、陕优412（2^＊，7＋8，5＋10）、绵阳89-47（2^＊，13＋16，5＋10）以及中优16、冀5099、绵优1号、绵优2号（1，17＋18,5＋10），占参试品种的44．4％。由Glu-3位点控制的低分子量谷蛋白亚基共12种图谱，小偃6号、PH82-2、陕253、80356、95鉴5104、郑农33等6个品种皆为“a，d，c”；由G1i-1位点控制的醇溶蛋白共12种图谱，小偃6号、PH82-2、陕优225、陕253、80356、中优16、95鉴5104等7个品种皆为“O，new，i”，即发现在Gli-B1位点是一个新等位基因。

Characterization of Glu-1、Glu-3 and Gli-1 Alleleic Variation of Storage Protein in 18 Quality Wheat Cultivars of China

The banding patterns of HMW-GS,LMW-GS and HMW gliadins of 18 quality wheat cultivars(lines) were analyzed using two-step SDS-PAGE and a modified A-PAGE to provide information for wheat quality breeding.14 HWM-GS band patterns were encoded by Glu-1 locus,61.1% and(11.1%) of them were 1 and 2~* in Glu-A1,27.8%,27.8%,22.2% and 5.6% of them were 14+15,7+8,17+18,13+16 in Glu-B1,55.6% of them were 5+10 in Glu-D1,respectively.Cultivars with positive subunits in all three Glu-1 locus are Shaan 451(1,7+8,5+10),95Jian5104(1,14+15,5+10),Shaanyou 412(2~*,7+8,5+10),Mianyang89-47(2~*,13+16,5+10),Zhongyou16,Ji5099,Mianyou 1 and Mianyou 2(1,17+18,5+10),and were about 44.4% of the tested cultivars.12 LMW-GS band patterns were encoded by Glu-3 locus.The patterns of 6 Cultivars of Xiaoyan 6,PH82-2,Shaan 253,80356,95Jian5104 and Zhengnong 33 were the same(a,d,c) at Glu-1.12 HMW gliadin band patterns were encoded by Gli-1 locus,the patterns of 7 Cultivars of Xiaoyan 6,PH82-2,Shaanyou 225,Shaan 253,80356,Zhongyou 16 and 95Jian5104 were the same(o,new,i),two new patterns at Gli-B1 locus were discovered which were different from the linked pattern h of dencoded by Glu-B3 of other cultivars.