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| 人参皂苷Rh2对肝癌SMMC-7721细胞增殖和细胞骨架的影响 | |
| 引用本文： | 王华,周滨,郭星,朱隽雅,程春,朱昌来,张天一.人参皂苷Rh2对肝癌SMMC-7721细胞增殖和细胞骨架的影响[J].中国病理生理杂志,2011,27(6):1226-1229. |
| 作者姓名： | 王华 周滨 郭星 朱隽雅 程春 朱昌来 张天一 |
| 作者单位： | 1. 南通大学 附属医院普外科, 江苏 南通 226001; 2. 南通大学 医学院, 江苏 南通 226001; 3. 南通大学 神经再生重点实验室, 江苏 南通 226001 |
| 基金项目： | 南通市基础研究科技计划资助项目，江苏省高等学校大学生实践创新训练计划资助项目 |
| 摘 要： | 目的: 探讨人参皂苷Rh2(Rh2)对人肝癌细胞SMMC-7721细胞生长和细胞骨架的影响。方法: 应用MTT法及流式细胞仪检测不同浓度Rh2对SMMC-7721细胞增殖的抑制及诱导凋亡作用;激光共聚焦显微镜观察细胞骨架肌动蛋白F-actin的分布状态;整装细胞电镜术观察细胞核基质-中间纤维系统的变化。结果: MTT法和流式细胞仪分析结果分别显示,40 mg/L Rh2 作用SMMC-7721细胞4 d时的抑制率及细胞凋亡率明显高于对照组及10、20 mg/L组,显著差异(P＜0.05);激光共聚焦显微镜下,SMMC-7721细胞经Rh2处理4 d,F-actin分布均匀,细丝排列整齐,数量增多;而对照组细胞F-actin细丝在细胞内排列紊乱,多呈斑点状分布。整装电镜结果显示,与对照组相比,实验组细胞的中间纤维丰富,均匀地满布细胞中,呈网架状连接的结构。结论: Rh2能有效抑制SMMC-7721细胞的增殖,增加SMMC-7721细胞的凋亡,并且诱导SMMC-7721细胞骨架发生变化。 |
| 关 键 词： | 肝肿瘤 诱导分化 人参皂苷Rh2 细胞骨架 |
| 收稿时间： | 2010-11-29 |
Effect of ginsenoside Rh2 on cell proliferation and cytoskeleton in hepatocarcinoma SMMC-7721 cells |
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| WANG Hua,ZHOU Bin,GUO Xing,ZHU Jun-ya,CHENG Chun,ZHU Chang-lai,ZHANG Tian-yi.Effect of ginsenoside Rh2 on cell proliferation and cytoskeleton in hepatocarcinoma SMMC-7721 cells[J].Chinese Journal of Pathophysiology,2011,27(6):1226-1229. | |
| Authors: | WANG Hua ZHOU Bin GUO Xing ZHU Jun-ya CHENG Chun ZHU Chang-lai ZHANG Tian-yi |
| Affiliation: | 1. Department of General Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China; 2. Medical College, Nantong University, Nantong 226001, China; 3. Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China. |
| Abstract: | AIM:To investigate the changes of cell growth and cytoskeleton in hepatocarcinoma SMMC-7721 cells treated with ginsenoside Rh2.METHODS: Cell viability and apoptosis under the conditions of ginsenoside Rh2 exposure at different concentrations were measured by MTT test and flow cytometry,respectively.The morphological changes of F-actin labeled with FITC-phalloidin were observed under confocal laser scanning microscope.The structures of nuclear matrix-intermediate fibre system were observed under transmission... |
| Keywords: | Liver neoplasms Induced differentiation Ginsenoside Rh2 Cytoskeleton |
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