| 人源性抗HBs可变区单链抗体基因的构建及核酸序列测定 |
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| 引用本文： | 任向荣,高辉,粟宽源,陈文吟,杨安钢,余宙耀.人源性抗HBs可变区单链抗体基因的构建及核酸序列测定[J].生物医学工程学杂志,2000,17(4):484-486. |
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| 作者姓名： | 任向荣 高辉 粟宽源 陈文吟 杨安钢 余宙耀 |
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| 作者单位： | 1. 中国人民解放军传染病中心医院,广州,510602
2. 第四军医大学,生化教研室,西安,710032 |
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| 摘 要： | 将通过噬菌体显示技术获得的一株人源性抗HBs Fab抗体基因重链和k轻链的可变区用编码柔性肽的Linker使其连接成单链，克隆入中间载体，并进行核酸序列测定，为其后的表达及双功能抗体的构建打下基础。
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| 关 键 词： | HBsAg 单链抗体 序列分析 乙型肝炎 |
Construction and Nucleotide Sequence Assay of Human Anti-HBs Variable Region Single-Chain Antibody Gene |
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| Ren Xiangrong,Su Kuanyuan,Gao Hui,Chen Wenyin,Yang Angang,Yu Zhouyao.Construction and Nucleotide Sequence Assay of Human Anti-HBs Variable Region Single-Chain Antibody Gene[J].Journal of Biomedical Engineering,2000,17(4):484-486. |
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| Authors: | Ren Xiangrong Su Kuanyuan Gao Hui Chen Wenyin Yang Angang Yu Zhouyao |
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| Affiliation: | Central Research Institution of Infectious Disease, 458th Hospital of PLA, Guangzhou 510602. |
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| Abstract: | The VH and Vk gene which were acquired using phage display technology have been combined by a linker encoding a flexible peptide (Gly4 Ser)3 to form a single-chain. Its nucleotide sequence was assayed after cloning the scFv gene into middle plasmid. The successful construction of single-chain gene lays the foundations for the subsequent expression and production of double functional antibody. |
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| Keywords: | HBsAg |
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