超声波辅助水解法制备高纯度大豆异黄酮工艺研究

以低纯度商品大豆异黄酮为原料,利用超声波辅助酸水解技术制备高纯度大豆异黄酮甙元产品。通过实验,比较系统深入研究超声波功率、超声波处理时间、反应温度、水解酸度等因素对水解效果影响,并最终得到超声波辅助水解最佳工艺参数。实验结果表明,超声波处理对大豆异黄酮甙水解有一定促进作用,超声波辅助水解大豆异黄酮最佳工艺条件为:超声波功率80W,水解时间1h,反应温度70℃,酸度为1M;通过超声波辅助水解提取后,所得产品纯度由原来25.3%提高至93.20%。

Study on isolating and purifying soy isoflavone aglycone byultrasound assisted acid hydrolysis

The high purification soy isoflavone aglycone was extracted from low purification soy isoflavone product by ultrasound-assisted acid hydrolysis.In this study,the factors which influence to the acid hydrolysis,such as ultrasound power,reaction time,reaction temperature,reaction system PH value and so on,are studied systematically and deeply.The experiment results indicate that the ultrasound have promoting function to isoflavone acid hydrolysis and the optimum extracting condition of ultrasound-assisted acid hydrolysis is ultrasound power 80W,reaction time 1h,reaction tempera- ture 70℃,concentration of acid 1M.The product purity is 93.2% by using ultrasound-assisted acid hydrolysis methods.