| 人皮肤成纤维细胞早期损伤后EDA-FN表达与损伤时间的关系 |
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| 作者姓名： | He M Yu S Xue AM Shen YW Chen L Gu YJ Zhao ZQ |
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| 作者单位： | 1. 复旦大学上海医学院法医系,上海,200032
2. 湖州市公安局,浙江,湖州,313000 |
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| 摘 要： | 目的探讨培养的人体皮肤成纤维细胞损伤后纤维连接蛋白EDA片段的表达情况与损伤时间的关系。方法培养人体皮肤成纤维细胞,利用单克隆抗体进行免疫组织化学染色及Western检测。结果培养中的人体成纤维细胞可以微量表达EDA,随损伤时间延长而表达量增加。结论EDA片段表达变化可以作为法医学早期损伤时间推断的比较理想的指标。
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| 关 键 词： | 纤维连接蛋白 EDA片段 损伤时间 成纤维细胞 |
| 文章编号： | 1004-5619(2007)02-0094-03 |
| 修稿时间： | 2007年1月16日 |
Expression of EDA-fibronectin in injured human skin fibroblast and its potential application in estimation of wound healing |
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| He M,Yu S,Xue AM,Shen YW,Chen L,Gu YJ,Zhao ZQ.Expression of EDA-fibronectin in injured human skin fibroblast and its potential application in estimation of wound healing[J].Journal of Forensic Medicine,2007,23(2):94-96,F0004. |
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| Authors: | He Meng Yu Song Xue Ai-Min Shen Yi-Wen Chen Long Gu Yun-Ju Zhao Zi-Qin |
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| Affiliation: | Department of Forensic Medicine, Shanghai Medical Collage, Fudan University, Shanghai 200032, China. |
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| Abstract: | Objetive To study the expression of EDA-FN variant in injured human skin fibroblast and its relation to wound healing.Methods Human skin fibroblast cells were separated from human skin and cultured and monoclonal antibody to fibronectin IST-9] was applied to amplify target segments.Results Trace amount of EDA-FN was detected in the culture cells.The expression was increased within 8 hours after the injury.Conclusions The expression of EDA-FN would be an ideal marker for estimation of wound healing |
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| Keywords: | FN EDA wound interval estimation fibroblast |
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