实验性蛛网膜下腔出血对脑微血管内皮细胞的作用研究

目的　建立实验性蛛网膜下腔出血后内皮细胞损伤模型 ,并观察血性脑脊液 (bloodycerebral spinalfluid ,BCSF)对培养的微血管内皮细胞的损害作用。方法　将体外培养的兔脑微血管内皮细胞分为对照组和BCSF刺激组 ,通过MTT比色法选择恰当孵育时间的BCSF作为刺激因素 ,观察病理形态学改变、细胞计数、流式细胞术细胞周期 ,分析评估内皮细胞损害和增殖状况。结果　与对照组比较 ,孵育 7天BCSF可使内皮细胞出现明显的病理形态学改变 ,贴壁细胞数量减少 ,MTT吸光度下降 (P <0 .0 1) ,G0 ～G1期细胞比例增高 (P <0 .0 1)。结论　BCSF可直接造成明显的兔脑微血管内皮细胞病理形态学损害 ,并抑制血管内皮细胞代谢和增殖。

Effect of experimental subarachnoid hemorrhage on microvessel endothelial cells of brain

Objective To establish an endothelial cell model in vitro following experimental subarachnoid hemorrhage,and study the cytoxic effect of bloody cerebrospinal fluid (BCSF)on endothelial cells.Methods Rabbit brain microvessel endothelial cells(BmvECs) were divided into a control group and a BCSF group.MTT assay was taken to evaluate the injury of BCSF incubated for different time on BmvECs in order to decide the suitable BCSF as pathogen.Morphological changes,cell counting,MTT assay,flow cytometric analysis were used to evaluate the cytoxic effects on BmvECs.Results BCSF could cause severe morphologic changes in BmvECs and decreased MTT assay,and increased cell proportion of G 0 -G 1 of BmvECs(P<0.01).Conclusion BCSF could cause direct injury on endothelial cells,including morphological changes and decreased proliferation.