苦参抗白血病作用及其机制

采用CFU GM和白血病CFU L集落培养、NBT/MTT还原试验和DNA的片段化分析法 ,观察了苦参对正常人骨髓、脐带血粒 巨噬系造血祖细胞 (CFU GM)和人急性髓系白血病HL 6 0细胞增殖、分化、凋亡的影响。结果表明 :5 ,10 ,15 ,2 0 μg·μl-1苦参均能明显抑制HL 6 0细胞增殖并诱导其凋亡 ,呈浓度 效应关系 ;其中 15 μg·μl-1苦参诱导HL 6 0细胞分化作用明显 ,且 5～ 15 μg·μl-1苦参对正常人造血祖细胞无明显的细胞毒作用。提示苦参在一定浓度范围内有选择性抗白血病效应。此结果为进一步研究和开发苦参并应用于肿瘤临床提供了实验依据。

The anti-leukemia effect of Sophora flavescens and its mechanism

By using CFU GM/CFU L colony assay, NBT/MTT reductant test and DNA fragmentation analysis, we studied the effects of Sophora flavescens(SF) on CFU GM proliferative ratio in human normal bone marrow/umbilical cord blood and on proliferation, differentiation and apoptosis in human acute myelogenous leukemia HL 60 cells. The results showed that 5,10,15,20?μg·μl -1 of SF significantly inhibited proliferation and induced apoptosis in the HL 60 cells in a dose dependent fashion. Fifteen μg·μl -1 of SF also induced differentiation in HL 60 cells. Furthermore, cytotoxic activity of SF(5～15?μg·μl -1 ) was not apparent on human normal hematopoietic progenitors(CFU GM). The results indicate that an appropriate concentration of SF has a selective antileukemic effect. Thus, these are important impetuses for further research of SF as an anticancer agent.