机械损伤对糙皮侧耳原基形成的影响

原基期是糙皮侧耳Pleurotus ostreatus发育进程的关键时期。本研究以糙皮侧耳菌株New 831为试验材料,通过对糙皮侧耳菌丝机械损伤,探讨机械损伤对糙皮侧耳原基诱发的影响。菌丝满板后,经过机械损伤处理,到原基期每隔4d取样一次,测定了这些样品处理组与对照组的活性氧及脂质过氧化水平,并且通过荧光定量分析了Po.WC-1、Po.WC-2、fst3和fst4基因的表达量。结果表明：机械损伤组和对照组的活性氧水平随时间的增加均呈现“先升后降”的趋势,机械损伤总体提高了活性氧的水平,并且机械损伤导致原基提前形成,表明活性氧水平的提高,可能促进了糙皮侧耳原基形成;荧光定量分析发现糙皮侧耳菌丝机械损伤处理后,Po.WC-1、Po.WC-2、fst3、fst4基因的表达量均发生变化,这些基因的变化表明它们可能参与调控糙皮侧耳原基形成;Po.WC-2和fst3基因在第8天时的显著上调表达可能促进了糙皮侧耳原基形成。研究结果表明机械损伤能够促进糙皮侧耳原基提前形成,这将为进一步研究原基形成过程中机械损伤的作用机制奠定基础。

Effects of mechanical damage on the primordium formation of Pleurotus ostreatus

Primordium stage is a key development stage of Pleurotus ostreatus. In this study, the effects of mycelial mechanical damage treatment on the primordium formation of P. ostreatus New 831 were reported. The mechanical damage treatment was carried out after the mycelia were over grown on the plate. Samples were taken every four days until the beginning of primordium stage, and the reactive oxygen species and lipid peroxidation levels in treated groups and control group were measured. The expression levels of Po.WC-1, Po.WC-2, fst3 and fst4 were analyzed by qRT-PCR. The results showed that the reactive oxygen species levels of both groups increased at first and then decreased. The level of reactive oxygen species of the treated groups was generally improved, and primordium formation was significantly advanced. These results indicated that the increase of reactive oxygen species level might promote the primordium formation of P. ostreatus. The expression levels of Po.WC-1, Po.WC-2, fst3 and fst4 changed after mechanical damage treatment, and qRT-PCR analysis indicated that they might be involved in the regulation of primordium formation. The significant up-regulation of Po.WC-2 and fst3 on day 8 may promote the formation of primordium. The mechanism of mechanical damage in the process of P. ostreatus primordium formation needs further study.