| 血小板源生长因子-AB对成纤维细胞周期及DNA合成的影响 |
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| 引用本文： | 董茂龙,陈璧,贾赤宇,汤朝武,曹云新.血小板源生长因子-AB对成纤维细胞周期及DNA合成的影响[J].第四军医大学学报,1999,20(11):S084-S086. |
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| 作者姓名： | 董茂龙 陈璧 贾赤宇 汤朝武 曹云新 |
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| 作者单位： | 1. 第四军医大学西京医院整形外科中心烧伤科陕西,西安,710033
2. 第四军医大学中心实验室,陕西,西安,710033 |
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| 摘 要： | 目的：探讨血小板源生长因子（ＰＤＧＦ）－ＡＢ在创面愈合及瘢痕增生过程中的作用机制；方法：取第３代～６代体外培养的人正常皮肤成纤维细胞（ＮｓＦｂ）及增生性瘢痕成纤维细胞（ＨＴｓＦｂ），经ＰＤＧＦ－ＡＢ作用后，用＾３Ｈ－ＴｄＲ参入法测定ＤＮＡ合成的变化，用汉式细胞术测定细胞周期的变化。结果：ＰＤＧＦ－ＡＢ作用后，两种细胞ＤＮＡ民均明显增加，Ｓ期细胞数百分比均明显增高，Ｇ１期细胞不断进入Ｓ期，但作用有划
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| 关 键 词： | 血小板源生长因子 流式细胞数术 细胞培养 3H-TdR参入 |
| 文章编号： | 1000-2790(1999)11-S84-S86 |
| 修稿时间： | :1999-06-2 |
The effect of platelet-derived growth factor-AB on cell cycle and DNA synthesis of fibroblasts |
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| DONG Mao-Long,CHEN Bi,JIA Chi-Yu,TANG Chao-Wu,CAO Yun-Xin.The effect of platelet-derived growth factor-AB on cell cycle and DNA synthesis of fibroblasts[J].Journal of the Fourth Military Medical University,1999,20(11):S084-S086. |
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| Authors: | DONG Mao-Long CHEN Bi JIA Chi-Yu TANG Chao-Wu CAO Yun-Xin |
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| Abstract: | AIM: To investigate the mechanism by which PDGF AB promotes wound healing and plays roles in scar forming. METHODS:The 3rd 6th passage normal skin fibroblasts (NsFb) and hypertrophic scar fibroblasts(HTsFb) were cultured with PDGF AB of different concentration, DNA synthesis was measured with 3H TdR incorporation, cell cycle was assayed with flowcytometry. RESULTS: After cultured with PDGF AB, DNA contents and the percentage of cells in S stage increased markedly, cells in G 1 stage entered S stage, but there were differences between NsFb and HTsFb. CONCLUSION: PDGF AB can promote wound healing by accelerating cell division and proliferation, and may play roles on hypertrophic scar. |
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| Keywords: | platelet derived growth factor flow cytometry 3H TdR incorporation |
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