房颤大鼠模型心室重构与心肌细胞钙稳态和心律失常的关联

摘要 目的：探讨房颤大鼠模型心室重构与心肌细胞钙稳态和心律失常的关联性。方法：将雄性Wistar大鼠随机平分为两组，各组8只，模型组采用乙酞胆碱-氯化钙混合液尾静脉注射法建立房颤动物模型，对照组注射同剂量的生理盐水，记录两组心室重构、心肌细胞钙稳态、心律失常情况并进行相关性分析。结果：模型组建模第2周与第4周的左室舒张末期内径（LVEDD）、左室收缩末期内径（LVESD）值都高于对照组（P<0.05）。模型组建模第2周与第4周的血清肌钙蛋白（cTnT）含量高于对照组（P<0.05）。模型组建模第2周与第4周心脏体外牵张性心律失常持续时间都高于对照组（P<0.05）。Pearson相关分析显示建模第2周与第4周的LVEDD、LVESD、cTnT与牵张性心律失常持续时间存在正相关（P<0.05）。结论：房颤大鼠伴随有心室重构与心肌细胞钙离子的大量释放，可增加牵张性心律失常持续时间，相关性分析结果表明：心室重构、心肌细胞钙稳态和心律失常存在显著正相关性。

Association of Ventricular Remodeling, Calcium Homeostasis and Arrhythmia in Atrial Fibrillation Rat Model

ABSTRACT Objective: To investigate the relationship between ventricular remodeling and cardiac muscle cell calcium homeostasis and arrhythmia in atrial fibrillation rat models. Methods: Male Wistar rats were randomly divided into two groups, each group was eight. The model group were injected into the tail vein of acetylcholine-calcium chloride to establish atrial fibrillation animal model. The control group were injected with the same dose of normal saline, and the ventricular remodeling, myocardial cell calcium homeostasis, arrhythmia of the two groups were recorded and given correlation analysis. Results: The left ventricular end-diastolic diameter (LVEDD) and left ventricular end-systolic diameter (LVESD) values in the 2nd and 4th weeks of modeling in the model group were higher than those of the control group (P<0.05). The level of serum troponin (cTnT) in the model group were higher in the 2nd and 4thweeks of modeling than in the control group (P<0.05). The duration of the cardiac stretch arrhythmia in the 2nd and 4th weeks of modeling in the model group was higher than that in the control group (P<0.05). Pearson correlation analysis showed that LVEDD, LVESD, cTnT were positive correlated with the duration of stretch arrhythmia in the 2nd and 4th week of modeling(P<0.05). Conclusion: Atrial fibrillation rats are accompanied by ventricular remodeling and the release of large amounts of calcium ions in cardiomyocytes, which can increase the duration of tractive arrhythmia. Correlation analysis results show that: ventricular remodeling, cardiac muscle cell calcium homeostasis and arrhythmia have a significant positive correlation.