| As2O3联合L-OHP对HepG2肝癌细胞株的体外抑制作用 |
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| 引用本文： | 吴兴军,崔林,刘建军,周向荣,刘兴祥,李阳.As2O3联合L-OHP对HepG2肝癌细胞株的体外抑制作用[J].实用癌症杂志,2010,25(4):333-336. |
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| 作者姓名： | 吴兴军 崔林 刘建军 周向荣 刘兴祥 李阳 |
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| 作者单位： | 江苏省姜堰市人民医院,225500 |
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| 摘 要： | 目的探讨As2O3联合L-OHP对HepG2肝癌细胞株的体外抑制作用。方法采用MTT法（四唑盐比色法）动态观察As2O3联合L-OHP对HepG2肝癌细胞的生长抑制作用,并采用两药相互作用系数（CDI）来评价两药相互作用的性质;应用流式细胞术（FCM）检测凋亡率和细胞周期分布。结果 As2O3与L-OHP联合应用,对HepG2肝癌细胞株的生长抑制、诱导凋亡作用均较相应的单药增强。流式细胞直方图上可见明显的＂凋亡峰＂,且As2O3使肝癌细胞周期阻滞于G2/M期,L-OHP使细胞周期阻滞于S期和G2/M期,两药合用使细胞周期阻滞于S期和G2/M期。结论中低浓度As2O3与L-OHP联合作用具有显著的协同效益,其主要机制可能是As2O3联合L-OHP诱导肝癌细胞凋亡作用及增强细胞周期阻滞作用。
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| 关 键 词： | 三氧化二砷 奥沙利铂 肝癌 细胞株 凋亡 |
Effects of Combination of Arsenic Trioxide and Oxaliplantin on Viability of Hepatocellular Carcinoma Cell Line HepG2 in Vitro |
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| Affiliation: | WU Xing-jun,CUI Lin,LIU Jian-jun,et al.(Department of Oncology,Jiangyan People＇s Hospital,Jiangyan,225500 ) |
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| Abstract: | Objective To explore the inhibiting effect of Arsenic trioxide（As2O3）combined with Oxaliplantin（L-OHP） on hepatocellular carcinoma cell line HepG2.Methods The inhibiting effect on the cells growth was observed by MTT assay.The character of the two drugs interaction was estimated by coefficient of drug in interaction（CDI）.The cell cycle and apoptotic changes were studied by the flow cytometry（FCM）.Results The inhibiting and apoptosis inducing effect were significantly higher by the combination of As2O3 and L-OHP than by any drug used alone.The cell cycle of HepG2 cells was arrested in G2/M phase by As2O3,in S＋G2/M by L-OHP,and in S＋G2/M phase by As2O3 combined with L-OHP.Conclusion As2O3 combined with L-OHP in moderate-low concentration have synergistic effect in inhibiting the cell growth of HepG2 cell line possibly by inducing carcinoma cell apoptosis and enhancing the function of cell cycle arrest. |
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| Keywords: | As2O3 L-OHP Hepatocellular carcinoma Cell line Apoptosis |
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