河南烟草主要病毒发生种类及侵染类型分析

为进一步明确河南烟区主要病毒种类及侵染类型,2020年从河南9个烟区分别采集疑似烟草普通花叶病毒(TMV)、黄瓜花叶病毒(CMV)、马铃薯Y病毒(PVY)和烟草蚀纹病毒(TEV)侵染的烟草样本共407份,分别利用上述4种病毒的特异性引物进行单重RT-PCR病原检测。检测结果表明,河南烟区病毒病样本的总检出率为98.28%,其中,检出率最高的是CMV为89.19%,其余依次为TMV 63.88%、PVY 28.99%和TEV 24.32%。侵染类型分为单一病毒侵染和复合病毒侵染,单一病毒侵染率为23.34%,该侵染类型中CMV所占比例最高,为16.71%;复合病毒侵染率为74.94%,复合病毒侵染类型中CMV+TMV所占比例最高,为36.86%,其次分别为CMV+TMV+PVY和CMV+TEV+PVY两种侵染类型,侵染比例均是7.62%。因此,CMV是2020年河南烟区的优势病毒,田间侵染以复合病毒侵染为主,侵染类型主要有CMV+TMV、CMV+TMV+PVY及CMV+TEV+PVY。

Detection and Analysis of Tobacco Viruses and Infection Type in Henan Province

To further clarify the main virus species and infection type in Henan Province,407 samples were collected from nine tobacco-growing areas in Henan Province in 2020.The samples were suspicious of being infected by Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV),Potato virus Y (PVY) and Tobacco etch virus (TEV) respectively.Then four pairs of specific primers were used to detect viruses that infected tobacco samples by single RT-PCR.The results showed that the infection percentage of virus diseases in Henan tobacco-planting areas was 98.28%,in which CMV (89.19%) took the top spot followed by TMV (63.88%),PVY (28.99%) and TEV (24.32%).It also suggested that infection type included single infection and co-infection.What's more,single infection rate was 23.34%,in which CMV had the highest infection percentage with 16.71%.And co-infection percentage was 74.94% which was much higher than the single infection rate.Furthermore,there were three main co-infection types,including CMV+TMV with the highest infection percentage 36.86%,followed by CMV+TMV+PVY and CMV+TEV+PVY which both had an infection percentage of 7.62%.Therefore,CMV was the dominant virus in Henan Province in 2020.And the infection types were CMV+TMV, CMV+TMV+PVY and CMV+TEV+PVY,which indicated that co-infection types occurred seriously in tobacco field.