华南忍冬总酚酸提取工艺优化及体外抗氧化活性研究

以华南忍冬为原料,采用微波辅助提取华南忍冬总酚酸,在单因素实验基础上,结合Box-Behnken响应面设计,探讨乙醇浓度、液料比、提取温度、提取时间和微波功率对总酚酸含量的影响,优化华南忍冬总酚酸最佳提取工艺条件,并通过DPPH自由基清除作用和铁离子还原能力来评价华南忍冬总酚酸提取物的抗氧化活性。结果表明最佳提取工艺条件为乙醇浓度80%、液料比12:1 mL/g、提取温度70℃、提取时间30 min、微波功率400 W,总酚酸含量达到59.45±0.13 mg/g,与模型预测值57.76 mg/g相近。提取物DPPH自由基清除率IC₅₀值为21.26 μg/mL,铁离子还原能力EC₅₀值为39.37 μg/mL。本试验表明华南忍冬总酚酸提取物具有较好的抗氧化活性,为华南忍冬综合研究提供理论依据。

Optimization of Extraction Process of Total Phenolic Acids from Lonicera confusa and Its Antioxidant Activity

The microwave-assisted extraction of total phenolic acid from Lonicera confusa was studied. Based on the single factor experiment and box-behnken response surface design,the effects of ethanol concentration,liquid-material ratio,extraction temperature,extraction time and microwave power on the yield of total phenolic acid were discussed,and the optimal extraction conditions of total phenolic acid from Lonicera confusa were optimized. The antioxidant activity of total phenolic acid extract of Lonicera confusa was evaluated by DPPH radical scavenging assay and ferric ion reducing power assay. The results showed that the optimum extraction conditions were 80% ethanol concentration,12:1 mL/g liquid to material ratio,70℃ extraction temperature,30 min extraction time,400 W microwave power,the total phenolic acid content reached 59.45±0.13 mg/g,which was similar to the model prediction value of 57.76 mg/g. The IC₅₀ value of DPPH radical scavenging rate was 21.26 μg/mL,and the EC₅₀ value of ferric ion reducing power assay was 39.37 μg/mL. The results showed that the total phenolic acid extract of Lonicera confusa had good antioxidant activity,which provided theoretical basis for the development of Lonicera confusa.