黑豆添加微量元素发芽后对雌性大鼠更年期综合征的影响

目的:通过添加微量元素发芽提高黑豆的营养价值,并对黑豆添加微量元素发芽后对雌性大鼠更年期综合征的影响进行研究。方法:以健康雌性SD(Sprague-Dawley,SD)大鼠为实验动物,制备摘除卵巢的大鼠动物模型(去势动物模型),随机分成7组,即正常组、模型组、阳性药组、生豆组、清水发芽组、富微低组、富微高组,分别给药,考察黑豆添加微量元素发芽后对摘除卵巢的雌性大鼠生殖内分泌的影响。结果:与正常组相比,模型组的阴道角化上皮细胞含量极显著降低86%(P<0.01),白细胞含量极显著升高1233%(P<0.01),上皮细胞含量极显著降低96%(P<0.01),雌二醇(Estradiol,E₂)显著降低68%(P<0.05),卵泡刺激素(Follicle stimulating hormone,FSH)显著升高28%(P<0.05),成功建立模型。与模型组相比,生豆组、清水组、富微高组、富微低组都能极显著升高角化上皮细胞含量281%、362%、455%、466%(P<0.01);生豆组与模型组相比E₂极显著升高85%(P<0.01),FSH显著降低12%(P<0.05)。富微高组、富微低组与模型组相比FSH分别显著降低10%、14%(P<0.05);与模型组相比,富微高组与富微低组子宫内膜厚度明显增厚,腺体数量增多,病理性增生减少,炎性浸润减轻。与生豆组相比,清水组、富微低组、富微高组均能显著升高角化上皮细胞含量21%、46%、48%(P<0.05)。与清水组相比,富微低组、富微高组均能显著升高角化上皮细胞含量19%、22%(P<0.05)。富微高组与富微低组比角化上皮细胞含量没有显著性差异。结论:黑豆添加微量元素发芽后对角化上皮细胞含量有极显著的升高作用,能在一定程度上综合改善雌性大鼠更年期综合征症状。

Effects of Black Bean Germinated with Trace Elements on Menopausal Syndrome in Female Rats

Objective:The nutritional value of black bean was improved by adding trace elements to germinate black bean and the effects of black bean germinated with trace elements on menopausal syndrome in female rats were also studied. Methods:Healthy female SD rats were used as experimental animals to prepare ovariectomized rat models. They were randomly divided into seven groups:Normal group,model group,positive drug group,raw bean group,clear water germination group,low-dose group with trace element germination and high-dose group with trace element germination. The effect of black soybean germination with trace elements on reproductive endocrine of ovariectomized female rats was investigated. Results:The content of vaginal keratinized epithelial cells in model group was significantly lower than that in normal group by 86%(P<0.01),white blood cells content was significantly increased by 1233%(P<0.01),epithelial cells was significantly decreased by 96%(P<0.01),estradiol(E₂)was significantly increased by 68%(P<0.05),follicle stimulating hormone(FSH)was significantly increased by 28%(P<0.05).All above indicated that the model was established successfully. Compared with the model group,the content of keratinized epithelial cells in raw bean group,clear water group,high-dose group and low-dose group were significantly increased by 281%,362%,455% and 466%(P<0.01). Compared with the model group,the content of E₂ in raw bean group was significantly increased by 85%(P<0.01),and the FSH was significantly decreased by 12%(P<0.05). Compared with the model group,the FSH of the high-dose group and the low-dose group with trace element germination was decreased by 10% and 14%,respectively(P<0.05). Compared with the model group,the endometrial thickness of the high-dose group and the low-dose group was significantly thicker,the number of glands increased,the pathological proliferation decreased,and the inflammatory infiltration decreased. Compared with raw bean group,the contents of keratinized epithelial cells in clear water group,low-dose group and high-dose group were significantly increased by 21%,46% and 48%(P<0.05). Compared with clear water group,the content of keratinized epithelial cells increased by 19% and 22% in both low-dose group and high-dose group(P<0.05).There was no significant difference in keratinized epithelial cell content between high and low groups. Conclusion:The addition of trace elements to black soybean germinated significantly increased the content of keratinized epithelial cells,which could improve the symptoms of climacteric syndrome in female rats to a certain extent.