牦牛曲拉源乳酸菌的耐受性与抑菌性能比较

以源于牦牛曲拉的6株益生乳酸菌为研究对象,比较了耐酸能力、耐胆盐能力、耐渗透压能力和抑菌性能。结果表明:6株乳酸菌菌株的耐酸能力、耐胆盐能力、耐渗透压能力和抑菌性能各不相同。菌株G2、G4、Q1对酸性环境的耐受性最好,G2和G4的耐胆酸盐的能力最强,G2、G4、Q1对高渗透压的耐受性较强,所有菌株在模拟胃肠液环境下的存活率均在90%以上;菌株Q2对大肠杆菌的抑菌效果最强,菌株Q1次之;菌株Q1和Q2对金黄色葡萄球菌的抑制效果较好,其次是菌株G2;对沙门氏菌抑菌作用最强的为菌株Q2,抑菌能力最差为G3;对单增李斯特菌抑菌作用最强的为菌株G4。研究结果可为牦牛曲拉源益生乳酸菌的应用提供理论依据。

Comparison of Tolerance and Antibacterial Activity of Lactic Acid Bacteria from Yak Qula

Six strains probiotic lactic acid bacteria originated from yak Qula were used as the research objects,and their acid resistance,bile salt resistance,osmotic pressure resistance and inhibition ability were compared. The results showed that the six kinds of strains of lactic acid bacteria had different acid resistance,bile salt resistance,osmotic pressure resistance and inhibition ability. Strains G2,G4 and Q1 were best tolerant to acidic environments. Strains G2 and G4 had the strongest cholate tolerance. Strains G2,G4,and Q1 had strong resistance to high osmotic pressure. The survival rate of all strains in simulated gastrointestinal fluid were over 90%. Strain Q2 had the strongest antibacterial effect against Escherichia coli,followed by the strain Q1.Strain Q1 and Q2 had a better inhibitory effect on Staphylococcus aureus,followed by strain G2.Strain with the strongest antibacterial activity against Salmonella was strain Q2,and the worst antibacterial ability was strain G3.The strongest antibacterial activity against Listeria monocytogenes was the strain G4.The research results can provide a theoretical basis for the application of probiotic lactic acid.