呋喃西林代谢物酶联免疫吸附测定方法的建立及性能测定

建立动物源性食品中呋喃西林代谢物酶联免疫吸附测定方法。采用自制的呋喃西林代谢物抗原、抗体为基础原料,通过优化反应条件,建立间接竞争酶联免疫吸附试验(enzyme-linked immunosorbent assays,ELISA)检测方法,并对方法的性能进行测定。结果表明:建立的检测方法在0.03μg/L^2.43μg/L范围内呈线性,检测灵敏度为0.03μg/kg,鱼肉组织样本、虾肉组织样本的检测限分别为0.236、0.229μg/kg,回收率均在75%~120%之间,批内、批间变异系数均在15%以下,通过与标准方法液相色谱-串联质谱(high performance liquid chromatography-mass spectrum/mass spectrum,LC-MS/MS)对比验证,两种方法用于鱼肉样本的检测呈现较好的相关性,相关系数R2为0.9944。建立的呋喃西林代谢物酶联免疫吸附测定方法的灵敏度、准确度和精密度等参数均符合我国动物源性食品中兽药残留检测方法的规定。

Establishment of Enzyme-Linked Immunosorbent Assay for Determination of Furacillin Metabolites and Its Properties

To establish an enzyme-linked immunosorbent assay for the determination of furacillin metabolites in animal derived food.Using the furacillin metabolite antigen and antibody as the basic raw materials,indirect competitive enzyme-linked immunosorbent assays(ELISA)method was established and its performance was measured by optimizing the reaction conditions.The established detection method was linear within the range of0.03μg/L-2.43μg/L.The detection sensitivity was 0.03μg/kg and the detection limits of fish tissue samples and shrimp tissue samples were 0.236μg/kg and 0.229μg/kg,respectively.The recoveries were between 75%and 120%,the variation coefficient between batch and batch were below 15%,compared with standard method of high performance liquid chromatography-mass spectrum/mass spectrum(LC-MS/MS),two methods used in the detection of fish samples were showing good correlation,the correlation coefficient R2 of 0.9944.The sensitivity,accuracy and precision of the established method for the determination of furacillin metabolites by enzyme-linked immunosorbent were in line with the requirements of the method for the detection of veterinary drug residues in food of animal origin in China.