桦菌芝多糖抗氧化性及抑菌活性研究

以桦菌芝为原料,采用水提取乙醇沉淀法提取桦菌芝多糖,分析其清除DPPH自由基和ABTS自由基能力,并用滤纸片法测定不同浓度桦菌芝多糖对大肠杆菌、金黄色葡萄球菌、枯草杆菌和四联球菌的抑菌活性及最低抑菌浓度(MIC)。结果表明,桦菌芝多糖对DPPH自由基和ABTS自由基均具有消除作用,当浓度为1.0mg/mL时,桦菌芝多糖对DPPH自由基和ABTS自由基的清除能力均超过BHT对照品,但较弱于VC对照品;不同浓度桦菌芝多糖的抗氧化活性具有显著性差异。桦菌芝多糖对4种受试菌均具有抑制作用且存在显著性差异,其中对大肠杆菌的抑制活性最强。大肠杆菌、金黄葡萄球菌、枯草杆菌和四联球菌的最小抑菌浓度分别为1.25,1.25,2.50,5.00mg/mL。综上,桦菌芝多糖具有较好的抗氧化及抑菌活性,且抗氧化及抑菌活性随多糖浓度的增大而逐渐加强。

Study on antioxidant and antibacterial activities ofpolysaccharides in Phropolyporus fomentarius

In this study, the water-extracted ethanol precipitation method was used to extract Polysaccharides in Pyropolyporus Formentarius (PFPS), and the capacities of scavenging DPPH and ABTS were analyzed. Different concentrations of PFPS were used to detect the resistant abilities against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, and Micrococcus tetragenus, and the relative minimum inhibitory concentration (MIC) were also determined. The results showed that PFPS could eliminate DPPH and ABTS. When the concentration of PFPS was 1.0 mg/mL, the ability of scavenging DPPH and ABTS exceeded that of the BHT control, while was weaker than the VC reference substance. The antioxidant activity of PFPS with different concentrations was significantly different. PFPS had inhibitory effects on the four tested bacteria and there were significant differences, and the strongest inhibitory activity was resistant against E. coli. The minimum inhibitory concentrations of E. coli, S. aureus, B. subtilis, and M. tetragenus were 1.25, 1.25, 2.50, 5.00 mg/mL, respectively. In summary, PFPS had good antioxidant and antibacterial activities, with gradually increasing associated with the polysaccharide concentration.