广西融水县苗族六个短串联重复序列基因座的研究

目的了解广西融水县苗族人群无关个体的6个短串联重复序列基因座D7S820，D13S317，D16S539，HUMCSF1PO，HUMTPOX，HUMTH01遗传多态性分布情况，获得相应多态位点的群体遗传学数据。方法用枸橼酸钠抗凝法采集血样，酚-氯仿抽提法提取DNA，应用AmpeFSrR Identifiler^TM荧光标记复合扩增技术和ABI Prism 3100型遗传分析仪进行检测分析。结果6个基因座D7S820，D13S317，D16S539，HUMCSF1PO，HUMTPOX，HUMTH01分别检测出7、8、6、7、5、7个等位基因，基因型的分布符合Hardy—Weinberg平衡定律（P〉0．05），其累积个人识别力0．999995，累积非父排除率0．9959，多态信息总量0．9987。结论上述6个基因座具有高度多态性和遗传稳定性，符合孟德尔遗传规律，可为广西苗族的民族遗传学研究和法医学鉴定的提供基础数据。

Genetic polymorphism of 6 short-tandem repeat loci in Miao minority group of Rongshui county in Guangxi province

OBJECTIVE: To investigate the distributions of six short-tandem repeat (STR) loci, namely D7S820, D13S317, D16S539, HUMCSF1PO, HUMTPOX and HUMTH01, in Miao minority group at Rongshui county in Guangxi province and construct the relevant genetic database. METHODS: Sodium-citrated blood specimens were collected from 208 healthy unrelated Miao individuals in Rongshui county. The DNAs from the specimens were extracted with phenol-chloroform method; AmplFSTR Identifier PCR Amplification Kit was used to amplify the extracted DNAs, and 3100 Genetic Analyzer was used to analyze and screen the amplified products. RESULTS: In this study, 7, 8, 6, 7, 5, 7 alleles were observed at the 6 STR loci respectively. The expected distribution of genotype accorded with Hardy-Weinbery equilibrium. The total discrimination power, cumulative paternity exclusion power and total polymorphism information were 0.999995, 0.9959 and 0.9987 respectively. CONCLUSION: The results demonstrate that these 6 STR loci are of high polymorphism and hereditary stability and are in accord with Mendel's law. The data obtained are valuable in population genetics research, forensic application, and individual identifications.