| 高效液相色谱法测定复方地黄合剂中黄芩苷的含量 |
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| 引用本文： | 张武标,沈阳,王申东,刘兆康.高效液相色谱法测定复方地黄合剂中黄芩苷的含量[J].儿科药学杂志,2012,18(10):41-43. |
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| 作者姓名： | 张武标 沈阳 王申东 刘兆康 |
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| 作者单位： | 1. 上海电力医院,上海,200050
2. 上海市儿童医院,上海,200040 |
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| 摘 要： | 目的:建立复方地黄合剂中黄芩苷的含量测定方法.方法:采用高效液相色谱( HPLC)法,色谱柱为Calesil C18(5μm,250 mm×4.6 mm),流动相为甲醇-0.2％磷酸水溶液(51∶49),检测波长278 nm.结果:黄芩苷在10～ 80 μg/mL浓度范围内与峰面积呈良好的线性关系(r=0.999 9),低、中、高3种浓度的平均回收率分别为99.65％、100.87％、99.23％,RSD分别为0.43％、0.44％、0.44％.结论:该法简便、准确、重复性好,可用于复方地黄合剂的质量控制.
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| 关 键 词： | 复方地黄合剂 高效液相色谱法 黄芩苷 |
Determination of the Content of Baicalin in Compound DiHuang Oral Solution by HPLC |
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| Zhang Wubiao
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Shen Yang
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Wang Shendong
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Liu Zhaokang.Determination of the Content of Baicalin in Compound DiHuang Oral Solution by HPLC[J].Journal of Pediatric Pharmacy,2012,18(10):41-43. |
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| Authors: | Zhang Wubiao Shen Yang Wang Shendong Liu Zhaokang |
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| Affiliation: | 1.Shanghai Electric Power Hospital,Shanghai 200040,China;2.Children’s Hospital of Shanghai,Shanghai 200040,China) |
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| Abstract: | Objective: To establish a method to determine the content of baicalin in Compound Dihuang Oral Solution.Methods: HPLC method was used to carry on the determination.Calesil C18 column(5 μm,250 mm×4.6 mm)was used,mobile phase was methanol-0.2% phosphoric acid(51∶49),detection wavelength was 278 nm.Results: Baicalin sample size located 10~80 μg/L,favorable linear correlation(r=0.999 9) was showed.The average recovery rates of low,moderate and high concentration were 99.65%,100.87% and 99.23% respectively and RSD was 0.43%,0.44% and 0.44% respectively.Conclusions: The method is convenient and accurate;meanwhile,it also has a good reproducibility,may be used for quality control of baicalin. |
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| Keywords: | Compound dihuang oral solution HPLC Baicalin |
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