| 应用微卫星DNA标志研究我国大劣按蚊群体的遗传差异 |
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| 引用本文： | 王冬,马雅军,周红宁.应用微卫星DNA标志研究我国大劣按蚊群体的遗传差异[J].中国媒介生物学及控制杂志,2010,21(2):98-101. |
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| 作者姓名： | 王冬 马雅军 周红宁 |
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| 作者单位： | 1. 第二军医大学病原生物学教研室,上海,200433
2. 云南省寄生虫病防治研究所 |
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| 基金项目： | 国家自然科学基金委员会-云南省人民政府联合基金 |
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| 摘 要： | 目的应用微卫星DNA分子标志阐明我国大劣按蚊（广义）群体的遗传差异水平。方法研究样本包括经分子鉴别的大劣按蚊（海南省实验室品系和海南省毛阳野生群体）和大劣按蚊D种（云南省江城和云南省勐腊野生群体）,扩增10个多态的微卫星DNA位点,用Arlequin软件分析和计算相关指标。结果对89个大劣按蚊样本进行微卫星位点的扩增,等位基因数范围为1～32个,平均值为3.60～25.20,并非所有微卫星位点在所有群体中均呈现多态性。群体的平均期望杂合度和观察杂合度的范围分别为0.49～0.72和0.36～0.58,两者在海南省群体中均为最低。成对群体间的FST值为负值或很小,提示群体间未出现遗传分化。分级AMOVA计算结果显示,大劣按蚊群体内的变异占总变异的103.29%,种内变异为负值（-3.97%）,种间变异仅占总变异的0.67%。结论大劣按蚊与大劣按蚊D种内和种间遗传差异均非常小,微卫星DNA的变异主要在个体间。
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| 关 键 词： | 大劣按蚊（广义） 微卫星DNA 群体遗传差异 |
Study on the genetic differences among Anopheles dirus sensu lato (Diptera:Culicidae) in China using microsatellite DNA markers |
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| WANG Dong,MA Ya-jun,ZHOU Hong-ning.Study on the genetic differences among Anopheles dirus sensu lato (Diptera:Culicidae) in China using microsatellite DNA markers[J].Chinese Journal of Vector Biology and Control,2010,21(2):98-101. |
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| Authors: | WANG Dong MA Ya-jun ZHOU Hong-ning |
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| Affiliation: | WANG Dong1,MA Ya-jun1,ZHOU Hong-ning2. 1 Department of Pathogen Biology,Second Military Medical University,Shanghai 200433,China,2 Institute of Parasitic Disease Control of Yunnan Province |
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| Abstract: | Objective Microsatellite DNA molecular markers were used in the present study to identify the genetic diversity of Anopheles dirus sensu lato in China. Methods Molecularly identified,An. dirus (laboratory strains in Hainan province and wild population from Maoyang,Hainan) and species D of An. dirus (wild populations from Jiangcheng and Mengla,Yunnan province) constituted the samples,of which ten polymorphic microsatellite DNA loci were amplified and calculation of relevant index were performed using Arlequi... |
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| Keywords: | Anopheles dirus(sensu lato) Microsatellite DNA Population genetic difierences |
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