PTEN及Bcl-2蛋白在膀胱移行细胞癌中的表达及其意义

目的：探讨PTEN及Bcl-2蛋白在膀胱移行细胞癌的表达规律及其临床意义。方法：应用免疫组织化学链亲和素-生物素-霉复合物（SP）方法检测43例膀胱移行细胞癌和7例正常黏膜组织中PTEN及Bcl-2蛋白的表达．分析二者的表达与膀胱癌病理参数的关系。结果：（1）G1、G2、G3肿瘤PTEN表达阳性率分别为85．7％、80．4％、73．3％，浸润性肿瘤和表浅性肿瘤表达阳性率分别为70．4％和93．8％，说明PTEN表达阳性率与肿瘤病理分级、临床分期有关。（2）G。、G2、G3肿瘤Bcl-2表达阳性率分别为14．2％、57．14％、66．67％，浸润性肿瘤和表浅性肿瘤表达阳性率分别为59．3％和43．8％，说明Bcl-2表达阳性率与肿瘤病理分级、临床分期有关。（3）随着肿瘤恶性程度的增高和临床分期进展，PTEN蛋白阳性率呈下降，Bcl-2表达呈增高趋势，二者表达呈负相关关系。结论：PTEN的抑癌作用可能与Bcl-2有关．二者的异常表达在膀胱移行细胞癌的发生、发展过程中起重要作用。二者同时检测有助于判断预后。

Expression of PTEN and Bcl-2 Protein in Bladder Transitional Carcinoma and Its Clinical Significance

Objective: To investigate the expression of PTEN and Bcl-2 protein in bladder transitional cell carcinoma (TCC) and its clinical significance. Methods: The expression of PTEN and Bcl-2 protein were detected by S-P immunohistochemistry technique in 43 cases of TCC and 7 cases of normal bladder mucosa. The relationship between PTEN and Bcl-2 expression were analyzed. Results: (1) There was positive expression in all 7 cases of normal bladde mucosa, the positive rates of PTEN expression in different grade tumor were respectively 85.7% (G1), 80.4% (G2) and 73.3% (G3). The positive rate of PTEN expression had a relationship with tumor grade and stage. (2) The positive rates of Bcl-2 expression in different grade tumor were respectively 14.2% (G1), 57.14% (G2)and 66.67% (G3). The positive rates of PTEN expression had a relationship with tumor grade and stage. (3) With the progress of the tumor grade and clinical stage, the positive rate of PTEN protein in TCC decreased, while that of Bcl-2 increased. PTEN protein expression was negatively correlated with Bcl-2 expression. Conclusion: The anticancer effects of PTEN may be related with Bcl-2 expression, the abnormal expression of PTEN and Bcl-2 may play an important role in the development of TCC. Detection of both proteins might be helpful in prognostic of TCC.