茶多酚对奥氮平诱导肥胖大鼠的减肥作用及其机制

目的探讨茶多酚对奥氮平诱导肥胖大鼠的减肥作用及其机制。方法 SD雌性大鼠50只,随机分为正常对照组(10只)和奥氮平诱导肥胖模型组(40只)。肥胖模型组于黑暗时相前1h奥氮平1.2 mg·kg~(-1)·d~(-1)灌胃,正常对照组给予等容量蒸馏水,连续2 wk。于wk 3开始,再将肥胖模型大鼠随机分为4组:模型组、西布曲明(2.5 mg·kg~(-1)·d~(-1))组、茶多酚低(150 mg·kg~(-1)·d~(-1))剂量组和茶多酚高(300 mg·kg~(-1)·d~(-1))剂量组,每组10只。连续给药5 wk。观察大鼠体重、肾和子宫周围脂肪湿重,计算Lee's指数和脂肪系数,测定血清瘦素、脂联素水平以及超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与正常对照组相比,模型组大鼠体重和脂肪湿重及Lee's指数和脂肪系数均增加,血清瘦素水平升高,脂联素水平降低(P<0.05)。与模型组比较,茶多酚低、高剂量组大鼠体重和脂肪湿重降低,Lee's指数、脂肪系数降低,SOD活性增加,MDA含量减少(P<0.05)。与模型组比较,茶多酚高剂量组血清脂联素水平升高(P<0.05),瘦素水平无显著差异(P>0.05)。结论茶多酚对奥氮平诱导的肥胖大鼠有减肥作用,其作用机制可能与增强脂联素的调节和提高抗氧化能力有关。

Anti-obesity effects and its mechanism of tea polyphenols on obesity rats induced by olanzapine

AIM To study the anti-obesity effects of tea polyphenols(TP)on olanzapine induced weight gain in rats and its mechanism.METHODS Fifty female SD rats were randomized into 2 groups,10 rats in the normal control group and 40 rats in the obesity model group.The obesity model rats received olanzapine (1.2 mg·kg~(-1)·d~(-1))daily via gavage 1 h before the beginning of the dark-phase for 2 weeks,while the rats in the normal control group received an equivalent volume of distilled water.At the beginning of wk 3,the obesity model rats were randomized into 4 groups:model group(distilled water),sibutramine(2.5 mg·kg~(-1)·d~(-1)) group,low-dose(150 mg·kg~(-1)·d~(-1))group and high-dose(300 mg·kg~(-1)·d~(-1))group of TP,10 rats in each group,and the rats were administered with olanzapine continuously.TP and sibutramine were given by gavage for 5 weeks.The body weight and fat weight around the kidney and uterus were measured,and Lee's index and fat coefficient were calculated.The levels of serum leptin and adiponectin(ADP),superoxide dismutase (SOD)activity and malondialchehyche(MDA)content were tested respectively.RESULTS Compared with the normal control group,the body weight,fat weight,Lee's index,fat coefficient,the level of serum leptin were significantly increased and serum ADP decreased significantly in the model group(P<0.05).Compared with the model group,the body weight,fat weight,Lee's index,fat coefficient and the content of MDA decreased significantly,while the SOD activity increased significantly in the low-dose group and high-dose group of TP(P<0.05).Compared with the model group,the level of serum ADP increased significantly in the high-dose group (P<0.05),but there was no obvious difference in the level of leptin(P>0.05).CONCLUSION TP can reduce olanzapine induced weight gain in rats,which may be associated with the enhanced regulation of ADP and antioxidative ability.