| Vasculostatin慢病毒表达载体的构建 |
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| 引用本文： | 王伟,答嵘,王茂德,王拓,陈伟,李奇,祁磊.Vasculostatin慢病毒表达载体的构建[J].临床神经外科杂志,2014(1):38-40. |
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| 作者姓名： | 王伟 答嵘 王茂德 王拓 陈伟 李奇 祁磊 |
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| 作者单位： | [1]西安交通大学第一附属医院神经外科,西安710061 [2]西安交通大学第一附属医院检验科,西安710061 |
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| 基金项目： | 西安交通大学基本科研业务费（XJJ2010009） |
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| 摘 要： | 目的构建Vasculostatin慢病毒表达载体。方法通过基因合成方法获得Vasculostatin的完整序列,将此片段定向克隆到pL enti-CMV-EF1p-eG FP载体多克隆位点区,筛选重组质粒,采用磷酸钙方法将Lenti-CMV-Vasculostatin/EF1p-eG FP与载体pC MV-VSGS与pC MV-Gag-Pol共转染293T细胞,转染后收获上清离心纯化病毒并检测病毒滴度。结果成功构建Vasculostatin的慢病毒表达载体,检测病毒的滴度为2.5×107/ml。结论 Vasculostatin慢病毒表达载体为研究Vasculostatin对血管生成的抑制作用提供工具。
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| 关 键 词： | Vasculostatin 基因重组 慢病毒载体 |
Construction of lentiviral vector expressing Vasculostatin |
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| Affiliation: | WANG Wei, DA Rong, WANG Mao-de, et al. (Department of Neurosugery, the First Affiliated Hospital of Xi＇ an Jiaotong University, Xi＇ an 710061, China) |
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| Abstract: | Objective To construct a lentiviral vector expressing Vasculostatin. Methods Vaseulostatin sequence was acquired by gene synthesis and was directional cloned into pLenti-CMV- EFI p-eGFP. Lenti-CMV-Vasculostatin/EFlp-eGFP was cotransfected with pCMV-VSGS and pCMV- Gag-Pol into HEK 293T cells, the supematant was collected to purified virus, and virus titers were determined. Results Lentiviral vector expressing Vasculostatin was constructed successfully and virus titers were 2.5× 107/ml. Conclusion Lentiviral vector expressing Vasculostatin can be used in studying the anti-angiogenic potent of Vasculostatin. |
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| Keywords: | Vasculostatin gene recombination lentiviral vector |
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