痰中SP-D水平的改变在肺结核诊断中的价值探讨

目的     检测肺结核患者痰中肺表面活性物质相关蛋白D（SP-D）的水平及其变化，探讨其对肺结核诊断和鉴别诊断的价值。方法     采用ELISA方法检测100例肺结核患者、84例健康人痰中SP-D的水平及抗结核菌抗体，采用沉降法检测血沉，采用电阻法测定白细胞计数、中性粒细胞及淋巴细胞比例，采用Mantoux法进行PPD实验。结果    肺结核患者痰中SP-D的水平明显低于健康人（P<0.05），性别、年龄等因素对肺结核患者的SP-D水平没有明显影响（P>0.05）。 结论     测定痰中SP-D的水平对于肺结核的诊断和鉴别诊断具有参考价值。

Value of SP-D levels in the sputum in the diagnosis of pulmonary tuberculosis

Objective    To observe the level of surfactant protein-D(SP-D) in the sputum in pulmonary tuberculosis patients, and explore its value in the diagnosis of pulmonary tuberculosis. Methods     The SP-D level in the sputum was detected by ELISA in 100 samples from pulmonary tuberculosis patients and 84 samples from healthy subjects. The leucocyte count, neutrophilic granulocyte percentage and lymphocyte percentage were determined with the electric resistance method, erythrocyte sedimentation rates by the sedimentation process, and tubercle bacillus antibodies by ELISA. The PPD trial was executed with the Mantoux method. Results     Level of SP-D in the sputum in pulmonary tuberculosis was much lower than that in healthy subjects(P<0.05), and there was no obvious difference between pulmonary tuberculosis patients with different genders, ages and other factors (P>0.05). Conclusions     Detection of SP-D in the sputum is of great value in the diagnosis and differential diagnosis of pulmonary tuberculosis. The gender, age and other factors have nothing to do with the level of SP-D in the sputum in pulmonary tuberculosis.