多瘤病毒基因载量与出血性膀胱炎发生相关性的研究

目的研究多瘤病毒的基因载量与异基因造血干细胞移植出血性膀胱炎发生的关系。预防移植后出血性膀胱炎的发生。方法收集40例健康人和40例异基因造血干细胞移植及20例合并出血性膀胱炎患者的血液和尿液，合成多瘤病毒BKV、JCV和SV40基因引物，应用传统的PCR和EvaGreen染料荧光定量PCR法，分别检测血液和尿液多瘤病毒BKV、JCV和SV40DNA。结果40例健康人和40例异基因造血干细胞移植患者血液中BKV、JCV和SV40基因均为阴性；尿液中正常健康人BKV基因的检出率为15％（6／40），JCV基因的检出率为10％（4／40）。异基因造血干细胞移植患者BKV检出率为100％（40／40），JCV基因的检出率为12％（5／40），SV40基因均为阴性。20例出血性膀胱炎患者尿液中BKV基因载量均高于正常人和无出血性膀胱炎的异基因造血干细胞移植患者。结论正常健康人的泌尿系潜伏存在病毒BKV和JCV基因，异基因造血干细胞移植患者出血性膀胱炎的发生与BKV多瘤病毒DNA的载量有关。

Study the correlation between load of polyomavirus and hemorrhagic cystitis

OBJECTIVE: To study the correlation between polyoma virus load and hemorrhagic cystitis after allogeneic stem cells transplantation for prevention of hemorrhagic cystitis. METHODS: Blood and urine specimens were collected from 40 healthy persons, 40 patient with stem cells transplantation and 20 cases complicated with hemorrhagic cystitis for determination of VP1 gene of polyomaviruses BK virus (BKV)/Jamestown Canyon virus (JCV) and simian virus 40 (SV40) by polymerase chain reaction (PCR) and EvaGreen stain fluorescence quantitative assay. RESULTS: In the peripheral blood, all genes of BKV/JCV and SV40 were negative, while BKV gene in urine and blood from healthy persons and patient with stem cells transplantation was 15% (6/40) and 100% (40/40), respectively. The gene of JCV was positive in 10% (4/40) and 12% (5/40), the gene of SV40 was negative. CONCLUSION: Genes of BKV and JCV was detectable in urine specimens of healthy persons and there was a correlation between the load of polyomavirus and incidence of hemorrhagic cystitis.