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Study on nitrile-degrading microorganisms
作者姓名:Li Wenzhong  Zhang Hongyi  Yang Huifang
作者单位:Li Wenzhong,Zhang Hongyi and Yang HuifangInstitute of Microbiology,Academia Sinica,Beijing 100080,China
基金项目:This project was supported by National Natural Science Foundation of China.
摘    要:Two strains of bacteria were isolated from nitrile polluted soils, and identified as Corynebacterium boffmanii and Arthrobacter flavescens. Acetonitrile, propionitrile, butyronitrile and acrylonitrile were degraded by these bacteria to yield corresponding amides, carboxylic acids and ammonia. The nitrile-degrading abilities of these strains were investigated. The removal rates for the nitrile were nearly 100%, after these bacteria were grown in medium containing 10000 ppm of aceto-, propio-, or butyronitrile at 28 ℃ for 24h. When the reaction mkture consisting of 5000 ppm of above mentioned nitriles or acrylonitrile and 20g (dry cell) /L resting cells of the two strains in 0.06mol/L phosphate buffer (pH7.5) was incubated separately at 25 ℃ with moderate shaking for 15 or 45 min, the nitrile could be degraded completely. The optimum growth conditions for C.hoffmanii and A.flavescens were studied as well.

关 键 词:nitrile  microbial  degradation  microorganisms.
收稿时间:9/6/1990 12:00:00 AM

Study on nitrile-degrading microorganisms
Li Wenzhong,Zhang Hongyi,Yang Huifang.Study on nitrile-degrading microorganisms[J].Journal of Environmental Sciences,1991,3(3):91-97.
Authors:Li Wenzhong  Zhang Hongyi and Yang HuifangInstitute of Microbiology  Academia Sinica  Beijing  China
Affiliation:Li Wenzhong,Zhang Hongyi and Yang HuifangInstitute of Microbiology,Academia Sinica,Beijing 100080,China
Abstract:
Keywords:nitrile  microbial degradation  microorganisms  
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