E-cadherin 在鼻咽癌组织中的表达及临床意义*

摘要目的：检测鼻咽癌组织中上皮细胞钙黏蛋白（E-cadherin）的表达情况，探讨E-cad 与肿瘤侵袭、转移的关系。方法：收集临床 确诊的存档鼻咽低分化鳞癌石蜡标本40例，鼻炎标本20 例。将每个标本的4 长切片分别进行HE染色、免疫组化PV 二步法及 阴性对照。HE 确认病理类型，结合临床资病例料进行TNM分期，根据PV 二步法染色结果检测E-cadherin 的表达，所得结果用 SPSS17.0 进行统计学检验。结果：E-cadherin 在鼻咽癌组织对比中呈不同程度的降低（P=0.002），与性别、年龄无明显相关，与T 分 期（P=0.023）、淋巴结转移（P=0.001）、TNM 分期（P=0.000）显著相关。结论：1：E-cadherin 在鼻咽癌组和对照组的表达有显著的差 别，可能参与了鼻咽癌的发生发展。2：E-cadherin 的表达与肿瘤的淋巴结转移和病理分期有相关性，但与年龄和性别无相关性。 E-cadherin 的表达缺失是肿瘤远处转移的重要指标。3：E-cadherin 表达水平可能作为肿瘤侵袭，预测鼻咽癌颈部淋巴结隐匿性转 移的潜在肿瘤标志物。

Expression of E-cadherin in Nasopharyngeal Carcinoma and Their Clinical Significances*

ABSTRACT Objective:To investigate the expression of E-cadherin, as well as their correlations with the invasion and metastasis of nasopharyngeal carcinoma. Methods:40 cases of nasopharyngeal poorly differentiated squamous cell carcinoma and 20 cases of rhinitis tissues were selected, each of the four sections specimens were HE staining, immunohistochemical PV method and negative control. E-cadherin expression was detected according to the PV staining results. The results of statistical rests conducted by SPSS17.0. Results:E-cadherin was decreased or completely loss in nasopharyngeal carcinoma (P=0.002), lymph node metastasis (p=0.001)and TNM stage (P=0.000), not with gender and age.Conclusion: 1. The expression of E-cadherin in case and control group have a significant difference, which indicate that E-cadherin may have been involved in the tumor occurrence and development.2.E-cad- herin loss of expression, may be a biological maker in nasopharyngeal carcinoma. The levels of E-cadherin were useful molecular markers for evaluating malignancy degree and lymph node metastasis of in nasopharyngeal carcinoma.3.Expression of E-cadherin is an independent predictor of lymph node metastases in nasopharyngeal carcinoma, and it might be a tumor marker for occult lymph node metastases.