栀子有效部位的溃疡性结肠炎活性及其化学成分研究

目的研究栀子浸膏35%和70%乙醇洗脱部位对2,4,6-三硝基苯磺酸(TNBS)/乙醇诱导的大鼠溃疡性结肠炎(ulcerative colitis,UC)的治疗作用,并采用液质联用鉴定活性洗脱部位的化学成分。方法采用TNBS诱导大鼠UC模型,不同洗脱部均按生药量2.1 g·kg-1连续灌胃给药7 d,进行体质量测定,疾病活动指数(DAI)评分,结肠组织病理评分,比较各组大鼠结肠组织中髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6 (IL-6)、白细胞介素-1β(IL-1β)水平。采用液质联用鉴定药效较好部位的化学成分。结果与模型组相比,35%洗脱部位组体质量下降明显改善,DAI和组织病理评分降低,组织中MPO、NO、MDA、TNF-α、IL-6及IL-1β含量均降低,SOD含量升高(P <0.01)。液质联用共鉴定出19个化学成分,其中11个为环烯醚萜类成分。结论栀子35%洗脱部位对UC大鼠有明显治疗作用,环烯醚萜类成分可能是其发挥作用的物质基础。

Study on ulcerative colitis activity and its chemical constituents in effective part of Gardeniae Fructus

Aim To study the therapeutic effect of 35%and 70%ethanol elution sites of Gardeniae Fructus extract on 2,4,6-trinitrobenzene sulfonic acid(TNBS)/ethanol induced ulcerative colitis(UC)in rats,and to identify the chemical composition of the active elution site using mass spectrometry.Methods The UC model induced by TNBS was used in rats,and the different eluted parts were administered by gavage at a dose of 2.1g/kg for 7 days.Body weight measurement,disease activity index(DAI)score,and pathological score of colon tissues were compared.Myeloperoxidase(MPO),superoxide dismutase(SOD),malondialdehyde(MDA),nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin in mouse colon tissue-6(IL-6),interleukin-1β(IL^-1β)levels were compared among groups.Liquid-mass spectrometry was used to identify the chemical components of the parts with better efficacy.Results Compared with model group,the weight loss in 35%elution site group was significantly improved,the DAI and histopathology scores were markedly reduced,and the contents of MPO,NO,MDA,TNF-α,IL-6 and IL^-1βin tissues were apparently reduced.SOD content increased significantly(P<0.01).A total of 19 chemical components were identified by LC-MS,11 of which were iridoids.Conclusions The 35%elution site of Gardenia has obvious therapeutic effect on UC rats,and the iridoid component may be the material basis for its function.