| 高纯度青钱柳叶总三萜化合物精制研究 |
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| 引用本文： | 尹忠平,上官新晨,张月红,黎冬明,吴少福.高纯度青钱柳叶总三萜化合物精制研究[J].食品科技,2011(2):161-165. |
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| 作者姓名： | 尹忠平 上官新晨 张月红 黎冬明 吴少福 |
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| 作者单位： | 南昌大学食品科学与技术国家重点试验室;江西农业大学植物资源开发与利用研究室; |
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| 基金项目： | 江西省科技支撑计划项目(2009BSB09100); 江西省自然科学基金项目(2007GZN0214) |
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| 摘 要： | 目的:对青钱柳叶粗提总三萜化合物进行了纯化,以获得高纯度总三萜化合物,为分离三萜单体奠定基础。方法:大孔树脂动态吸附纯化;超声辅助固液萃取;活性炭脱色;高效预制薄板层析;L-B特征显色鉴定。结果:所选的4种供试大孔树脂中,AB-8对青钱柳三萜化合物具有较好的动态吸附、解吸性能,回收率可达70.89%,纯化后总三萜的纯度可达44.30%,适合于富集和纯化青钱柳叶粗提总三萜;以AB-8树脂动态吸附纯化总三萜,2.75 mg/mL的上样浓度、2 BV/h的上样流速、95%的乙醇洗脱剂、1.0 BV/h的洗脱流速为合适条件;经大孔树脂纯化的总三萜,采用乙酸乙酯进行固液萃取可获得较高的回收率和较好的纯化效果;以活性碳为脱色剂、乙酸乙酯为脱色溶剂微沸处理5 min,可获得较好的脱色效果;精制后的总三萜化合物为淡黄色粉末或片状物,有光泽,纯度可达94.61%。结论:采用AB-8树脂吸附、乙酸乙酯超声辅助固液萃取、活性炭脱色对青钱柳叶粗提总三萜进行纯化,可获得高纯度总三萜化合物。
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| 关 键 词： | 青钱柳 总三萜 精制 |
Purification of high purity total triterpenoids from Cyclocarya Paliurus leaves |
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| YIN Zhong-ping,SHANGGUAN Xin-chen,ZHANG Yue-hong,LI Dong-ming,WU Shao-fu.Purification of high purity total triterpenoids from Cyclocarya Paliurus leaves[J].Food Science and Technology,2011(2):161-165. |
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| Authors: | YIN Zhong-ping SHANGGUAN Xin-chen ZHANG Yue-hong LI Dong-ming WU Shao-fu |
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| Affiliation: | YIN Zhong-ping1,2,SHANGGUAN Xin-chen1,ZHANG Yue-hong2,LI Dong-ming2,WU Shao-fu2(1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,2.Laboratory of Plant Resources Exploitation & Utilization,Jiangxi Agricultual University,Nanchang 330045) |
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| Abstract: | Objective: In order to get high purity total triterpenoids and reference for separation of single triterpenoid,purification of crude total triterpenoids from Cyclocarya Paliurus leaves was studied in this paper.Methods: Purification by dynamic adsorption of macro-porous resin,solid-liquid extraction assisted by ultrasonic wave,discoloration by activated carbon,High Performance TLC,identification by Libermann-Buehard experiment.Results: Among 4 types of macro-porous resin,AB-8 had a good dynamic adsorption a... |
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| Keywords: | Cyclocarya paliurus total triterpenoids purification |
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