北京地区汉族人群ABO血型基因分型研究

本研究建立ABO基因分型技术，研究北京地区汉族人群中ABO基因型的分布，以了解该人群基因分布特点及规律。通过Multiplex·PCR·RFLP技术和PCR·SSP技术建立起稳定的ABO血型基因分型方法．应用该方法开展对北京地区汉族人群ABO基因分型的研究。研究结果表明，A102、O1、B为北京地区汉族人群中较为常见的等位基因；在北京汉族人群中A型以A102为主，占绝对优势；未检测到A201等位基因，但发现3例O2基因样本，此结果提示在北京汉族人群中存在O2基因。结论：通过研究初步了解了北京地区汉族人群ABO基因分型规律．为ABO血型系统的深入研究提供了参考依据。

ABO Genotyping of Han Population in Beijing

The aim of this study was to establish a diagnostic method for ABO genotyping and to investigate the distribution of ABO genotype in Beijing Han population so as to understand the distribution characteristics and regularity of ABO genotype. An ABO genotyping method was established by using multiplex-PCR-RFLP and PCR-SSP techniques, and the ABO allele frequency in Beijing Han population was investigated. The results showed that A102, O1 and B allele were more common genes in Beijing Han individuals. And A102 allele was predominant in the phenotype A group in this population. Three O2 alleles were found and no A201 allele was found while gene frequency investigation was performed. No A101A101, A101O2, A102O2, BO2 and O2O2 in this population were discovered. It is concluded that the primary regularity of ABO allele distribution in Beijing Han population is found through this study. It provides basic reference for further study of ABO types.