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霍乱弧菌O139菌毛微球疫苗制备初探 总被引:2,自引:1,他引:1
为寻求O139型霍乱弧菌菌毛疫苗研制的新途径,用高分子聚乳酸-聚乙二醇共聚物(DL-PLG)包裹毒素共调菌毛(TCP)抗的,进行免疫学研究,结果表明微轩比游离疫苗诱导的抗体水平高,其中小鼠血清IgG以皮下MS-TCP组最高,唾液中的sIgA以口服MS-TCP组最高,口服组主要诱导粘膜免疫,皮下组主要导全身性免疫,TCP是霍乱弧菌的共同抗原之一,可作为霍乱疫苗的候选抗原。 相似文献
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微球制剂临床应用进展 总被引:1,自引:0,他引:1
随着制剂技术的发展,微球制剂显示出了独特的优势,本文综述了药物微球的制备方法及临床应用现状. 相似文献
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目的:以脱乙酰壳聚糖为药物载体,制备了葛根素壳聚糖微球,考察葛根素壳聚糖微球在体外药物释放特性。方法以液体石蜡为油相,采用乳化-交联法制备葛根素壳聚糖微球,高效液相色谱法测定葛根素的含量。结果制备的葛根素壳聚糖微球形态圆整,大小均匀,表面光滑,平均粒径为9.56μm ,葛根素包封率为72.20%,平均载药量为17.82%。结论体外药物释放结果显示:以壳聚糖为载体,采用乳化-交联法制备的葛根素壳聚糖微球具有良好的缓释效果。 相似文献
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Microparticle-based vaccine delivery systems are known to promote enhanced immune responses to protein antigens and can elicit TH1-biased responses when used in combination with Toll-like receptor (TLR) agonists. It is important to understand the kinetics of the immune responses to microparticle-based protein vaccines in order to predict the duration of protective immunity and to optimize prime-boost vaccination regimens. We carried out a 10-week time course study to investigate the magnitude and kinetics of the antibody and cellular immune responses to poly(lactic-co-glycolic acid) (PLGA) microparticles containing 40 μg ovalbumin (OVA) protein and 16 μg CpG-ODN adjuvant (MP/OVA/CpG) in comparison to OVA-containing microparticles, soluble OVA plus CpG, or OVA formulated with Alhydrogel® aluminum adjuvant. Mice vaccinated with MP/OVA/CpG developed the highest TH1-associated IgG2b and IgG2c antibody titers, while also eliciting TH2-associated IgG1 antibody titers on par with Alhydrogel®-formulated OVA, with all IgG subtype titers peaking at day 56. The MP/OVA/CpG vaccine also induced the highest antigen-specific splenocyte IFN-γ responses, with high levels of IFN-γ responses persisting until day 42. Thus the MP/OVA/CpG formulation produced a sustained and heightened humoral and cellular immune response, with an overall TH1 bias, while maintaining high levels of IgG1 antibody equivalent to that seen with Alhydrogel® adjuvant. The time course kinetics study provides a useful baseline for designing vaccination regimens for microparticle-based protein vaccines. 相似文献
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《Journal of microencapsulation》2013,30(5):344-352
Matrix-enhanced delivery of cells is a promising approach to improving current cell therapies. Our objective was to create cell-laden composite microbeads that combine the attractive features of the natural polymers chitosan and fibrin. Liquid polydimethylsiloxane was used to emulsify a chitosan–fibrinogen solution containing suspended human fibroblast cells, followed by initiation of thrombin-mediated polymerization of fibrin and thermal/pH-mediated gelation of chitosan. Chitosan/fibrin weight percent (wt%) ratios of 100/0, 75/25, 50/50 and 25/75 were investigated. Microbead diameters ranged from 275?±?99?µm to 38?±?10?µm using impeller speeds from 600 to 1400?rpm. Fibroblasts remained viable on day 1 post-fabrication in all matrices, but cell viability was markedly higher in high-fibrin microbeads by day 8 post-fabrication. Cell spreading and interaction with the extracellular matrix was also markedly increased in high-fibrin matrices. Such composite microbeads containing viable entrapped cells have potential for minimally invasive delivery of cells for a variety of tissue repair applications. 相似文献
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Y. Watanabe T. Nakashima N. Yanagita 《European archives of oto-rhino-laryngology》1990,247(3):161-164
Summary The effects of sudden occlusion of the vein of the cochlear aqueduct (VCAQ) on the cochlear blood flow, endocochlear potential (EP), endolymphatic and perilymphatic fluid pressures (PE and PP) were studied in the guinea pig. Cochlear blood flow showed a sudden decrease, and EP began to drop within 90s, ranging from 50 to 70 mV in 5 of 11 animals studied, recovering to normal levels when the animals were placed on a continuous inhalation of carbogen. The PE and PP increased simultaneously (max. PE = 3.4 ± 1.4 mm Hg; max. PP = 2.5 ± 1.0 mm Hg) and returned to their initial values after 5 min. The EP was sustained within the normal range, even when there was an apparent decrease in cochlear blood flow (61.4 ± 8.4%). We believe that variations in EP following VCAQ occlusion were due to anatomical differences in collateral venous communications among the animals studied. Carbogen inhalation produced uniform recovery patterns, indicating that individual collateral vessel responses were eliminated. 相似文献