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1.
《Immunobiology》2022,227(6):152298
PLPPs (Phospholipid phosphatases) are widely expressed in different human tissues, regulate cell signal transduction, and are overexpressed in cancers such as gliomas, pancreatic adenocarcinoma, lung adenocarcinoma, and so on. As a member of the PLPP family, PLPP2 (phospholipid phosphatase 2) plays a vital role in the occurrence and development of breast cancer, but its mechanism is still unclear. Our research found that PLPP2 was overexpressed in breast cancer, and the higher expression level of PLPP2 showed a worse prognosis for breast cancer patients. Further analysis showed that overexpression of PLPP2 affected the expression of CDC34 (cell-division cycle 34), LSM7 (Like-Smith 7), and SGTA (small glutamine-rich tetratricopeptide repeat-containing protein alpha) through EMT (epigenetic-mesenchymal transition) related pathways to promote the occurrence and development of breast cancer. In vitro, silencing PLPP2 significantly reduced the proliferation, invasion, and migration abilities of human breast cancer cells MDA-MB-231. ER+ is a common subtype of breast cancer. Furthermore, we found that the overexpression of PLPP2 was significantly related to the poor prognosis of ER+ breast cancer. These results indicate that PLPP2 has value as a potential therapeutic target for breast cancer, especially for ER+ breast cancer.  相似文献   
2.
Radix Dipsaci, the dried root of Dipsacus asperoides C.Y. Cheng & T.M.Ai, has therapeutic effects on various disorders, and in particular, bone and joint disease. Despite such ethnomedicinal benefits, there is very little information regarding its in vivo toxicity or adverse effects. This study was conducted to evaluate the potential toxicity of the Radix Dipsaci water Extract (RD-wE) by using F344 rats. The RD-wE was administered orally to rats at doses of 0, 125, 250, 500, 1000, and 2000 mg/kg body weight (bw)/day for 13 weeks. During the treatment period there were no mortalities attributed to RD-wE. Moreover, no toxic effects were observed with regard to body weight, clinical pathology (hematology, clinical biochemistry, and urinalysis), and anatomic pathology (gross findings, organ weight, and microscopic examination). The changes related to the treatment were excessive salivation at the mouth and soft feces, observed in male and female rats at 1000 or 2000 mg/kg bw/day, but these were not accompanied by any microscopic correlate or other pathophysiological changes. Based on these results, the oral no-observed-adverse-effect level of the RD-wE was considered to be 2000 mg/kg bw/day in both genders, although the target organs were not determined under the current experimental conditions.  相似文献   
3.
目的 通过对比研究氧化苦参碱及其磷脂复合物大鼠离体肠吸收情况,考察磷脂复合物对氧化苦参碱肠吸收的影响。方法 采用大鼠离体外翻肠囊模型研究氧化苦参碱磷脂复合物的肠吸收特性。HPLC测定不同剂量的氧化苦参碱及氧化苦参碱磷脂复合物在大鼠十二指肠、空肠、回肠和结肠内的累积吸收量。结果 氧化苦参碱磷脂复合物在不同肠段的吸收较氧化苦参碱均有提高,低剂量组氧化苦参碱磷脂复合物在空肠、结肠处的吸收较氧化苦参碱有显著提高;中剂量组氧化苦参碱磷脂复合物在十二指肠、空肠、结肠处的累积吸收量较氧化苦参碱有显著提高;高剂量组氧化苦参碱磷脂复合物在空肠的吸收较氧化苦参碱有显著提高。结论 与氧化苦参碱相比,磷脂复合物对氧化苦参碱在大鼠肠道的吸收有显著的促进作用。  相似文献   
4.
目的:分析探讨帕金森病伴发轻度认知障碍患者血浆磷脂分子含量变化和意义。方法选择2012年3月-2014年3月该院治疗的帕金森病伴发轻度认知障碍患者50例,并选择同期在该院健康体检的50例和帕金森病无认知障碍的50例分别作为对照组A和对照组月,测定三组研究对象的血浆磷脂含量,并评定研究对象的智能状态,分析研究对象智能状态与血浆磷脂含量之间的关系。结果观察组MMSE得分、CDT得分以及MoCA得分均明显低于对照组A和对照组月(P<0.01);观察组的磷脂分子含量明显高于对照组A和对照组B(P<0.01);两组之间各项指标差异无统计学意义(P>0.05)。结论帕金森病伴发轻度认知障碍患者细胞膜受到了严重的损伤,血浆磷脂分子可作为新生物标记物,通过其含量变化可判断认知功能受损情况。  相似文献   
5.
研究葫芦素B胆盐磷脂混合胶束(Cu B-SDC/PLC-MMs) 在大鼠不同肠段的吸收动力学特征,并与葫芦素B混悬液进行对比。该研究采用外翻肠囊法制备离体肠吸收模型,高效液相色谱法测定葫芦素B的含量,研究Cu B-SDC/PLC-MMs在大鼠十二指肠、空肠、回肠、结肠的吸收状况,并考察不同质量浓度对肠吸收的影响。结果显示Cu B-SDC/PLC-MMs在不同肠段不同浓度下的吸收均为线性吸收(R2均大于0.9),符合零级吸收,且各肠段的吸收速率常数Ka随着药液中葫芦素B质量浓度的增加而增加,提示其吸收机制可能为被动吸收。Cu B-SDC/PLC-MMs在回肠段吸收最好,且各肠段的吸收均高于葫芦素B混悬液。磷脂胆盐混合胶束能显著增强葫芦素B的肠吸收,该肠吸收的研究为其进一步合理固化提供依据。  相似文献   
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9.
木犀草素制剂的研究进展   总被引:1,自引:0,他引:1  
木犀草素是一种具有多种药理活性的黄酮类化合物,但因其水溶性差及生物利用度低等限制了其临床应用。微粒给药系统、固体分散体、包合物、磷脂复合物、水凝胶等制剂技术的应用可提高木犀草素的生物利用度,综述了木犀草素制剂的研究进展,并阐述了其在细胞和动物实验中的应用,旨在促进其制剂开发及临床应用。  相似文献   
10.

Essentials

  • The prevalence of thrombocytopenia in patients with antiphospholipid syndrome is not well defined.
  • We studied triple positive patients with antiphospholipid syndrome and its catastrophic variant.
  • Prevalence of thrombocytopenia was 6% and 100% in patients who developed the catastrophic form.
  • In triple positive patients thrombocytopenia is low and platelets drop during the catastrophic form.

Summary

Background

Thrombocytopenia is the most common non‐criteria hematological feature in patients with antiphospholipid syndrome (APS). This condition is more common in patients with catastrophic APS (CAPS).

Objectives

To evaluate the prevalence of thrombocytopenia in a large series of high‐risk patients with APS, and to assess the behavior of the platelet count during CAPS.

Methods/Patients

This was a cross‐sectional study in which we analyzed the platelet counts of a homogeneous group of high‐risk APS patients (triple‐positive). Six of these patients developed a catastrophic phase of the disease, and the platelet count was recorded before the acute phase, during the acute phase, and at recovery.

Results

The mean platelet count in 119 high‐risk triple‐positive patients was 210 × 109 L?1. With a cut‐off value for thrombocytopenia of 100 × 109 L?1, the prevalence of thrombocytopenia was 6% (seven patients). No difference between primary APS and secondary APS was found. In patients who suffered from CAPS, a significant decrease from the basal count (212 ± 51 × 109 L?1) to that at the time of diagnosis (60 ± 33 × 109 L?1) was observed. The platelet count became normal again at the time of complete remission (220 ± 57 × 109 L?1). A decrease in platelet count always preceded the full clinical picture.

Conclusions

This study shows that, in high‐risk APS patients, the prevalence of thrombocytopenia is low. A decrease in platelet count was observed in all of the patients who developed the catastrophic form of the disease. A decrease in platelet count in high‐risk APS patients should be considered a warning signal for disease progression to CAPS.
  相似文献   
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