基于UPLC指纹图谱结合化学计量学评价不同产地盾叶薯蓣药材质量

目的建立盾叶薯蓣Dioscorea zingiberensis的UPLC指纹图谱,分析不同产地盾叶薯蓣质量特征的共有性和差异性,为盾叶薯蓣药材的质量评价提供科学依据。方法采用色谱柱Infinity Lab Poroshell 120 EC-C_(18)(150mm×2.1mm,2.7μm),以乙腈(B)-水(A)为流动相梯度洗脱,体积流量0.3mL/min,检测波长203nm,柱温30℃,建立65批不同产地盾叶薯蓣药材的UPLC指纹图谱;利用SPSS19.0和SIMCA14.1软件对不同产地的盾叶薯蓣药材进行质量评价及差异性分析。结果盾叶薯蓣药材UPLC指纹图谱共标定31个共有峰,指认出的5种皂苷类成分和其他未知成分均作为主要信息参与了盾叶薯蓣的质量表达,主成分载荷值的综合得分表明不同产地盾叶薯蓣药材的综合质量差异较大,主成分分析(PCA)结果显示不同产地盾叶薯蓣的化学质量特征存在差异,且各自聚为一类,其中湖北丹江口地区盾叶薯蓣与其他产地样品均存在较大差异;偏最小二乘法分析(PLS-DA)模型筛选出的5、28、11、12、29、18、16、31、13号色谱峰所代表的成分是造成盾叶薯蓣样品间差异的主要标志性物质,其中28、29号峰分别为盾叶新苷和三角叶薯蓣皂苷。结论本研究建立的盾叶薯蓣UPLC指纹图谱可以较为全面地表征其化学质量特征,指纹图谱的化学计量学分析结果为盾叶薯蓣质量标志物的筛选及质量标准的制定提供科学依据。     

瑞-姬氏染液复合染色法诊断卡氏肺孢子虫肺炎的研究

目的　制作颜色鲜明 ,结构清晰 ,易于观察的卡氏肺孢子虫包囊和滋养体染色标本。　方法　采用瑞 -姬氏染液复合染色法。　结果　染色后肺孢子虫包囊中的囊内小体有呈紫红色的细胞核和呈蓝色的细胞质 ,囊壁薄 ,淡蓝色 ,包囊各部分结构清晰可辨。滋养体有紫红色的细胞核和蓝色的细胞质。　结论　瑞 -姬氏染液复合染色法可在临床诊断和教学中推广应用     

Miltenberger blood group antigen subtype III (Mi.III) supports Wr(b) expression

Background and Objectives Miltenberger blood group antigens belong to the complex MNS system. Miltenberger antigen subtype III (Mi.III) was previously found to promote the expression of band 3. Here, we investigated whether the direct interaction between band 3 and Mi.III‐specific Gp.Mur (a glycophorin B‐A‐B hybrid) might affect the expression of related blood group antigens such as the Wright b (Wr^b) antigen. Materials and Methods (1) Band 3 genes of Mi.III+ and non‐Mi.III (control) donors were sequenced to determine the genotypes of the Wright antigens. (2) The expression levels of Wr^b, glycophorin A (GPA) and band 3 in Mi.III and the control erythrocytes were quantitatively assessed by flow cytometry. Results Mi.III erythrocytes expressed 22·5 ± 6·6% more Wr^b antigen than the control cells. The increase in Wr^b in Mi.III cells was independent of their GPA levels. Conclusion The elevated Wr^b levels in Mi.III RBCs were likely linked to their higher band 3 levels. Higher band 3 densities on the Mi.III+ cell surface conceivably could drive complex formation between band 3 and GPA/Gp.Mur, thereby increasing the expression of Wr^b.     

660例关节腔积液有形成分分析

目的:探讨关节腔积液有形成分分析在骨关节病中的诊断价值。方法:收集我院2017—2018年送检的660例关节腔积液,用普通光学显微镜及偏光显微镜观察关节腔积液有形成分。结果:痛风患者尿酸钠结晶或尿酸钠吞噬细胞阳性率为73.56%(192/261)。660例关节腔积液中,赖特细胞检出率为4.85%(32/660);脂肪滴检出率为2.73%(18/660);焦磷酸钙结晶和噬菌细胞检出率均为1.21%(8/660);狼疮细胞和橙色血质结晶检出率均为0.45%(3/660)。结论:关节腔积液有形成分分析在骨关节疾病的诊断和鉴别诊断中能提供重要线索和有力证据,帮助临床医生合理用药,使广大关节骨病患者早治疗早康复。     

瑞氏染液混配机的设计

目的设计一种可替代传统人工操作的瑞氏染液混配机。方法瑞氏染液混配机由混配罐、水箱、电机、电控箱和基座等构成。工作模式采用动力电机减速驱动混配罐翻转,借助于混配罐内壁摩擦撞击和研磨材料碰撞,使粉粒状瑞氏色素粉碎分散溶解成均一的极细小的微粒以至混配成瑞氏染液。通过实际配液和染色比较瑞氏染液混配机配液与人工配液的差异。结果瑞氏染液混配机混配的染液色素颗粒微细,质量优良,染色效果好,便于标本片的会诊和图像采集,避免了人工配制造成的染液质量不稳定和甲醇的毒副作用。结论该设计装备结构简单,设计合理,配液效率高,是一种特别适宜配制瑞氏染液的实验室实用新型设备。     

Olfactory Neuroblastoma

Few neoplasms are unique to the sinonasal tract, but sinonasal undifferentiated carcinoma and olfactory neuroblastoma are malignant tumors which require unique management. Due to the rarity of these tumors, practicing pathologists are not always aware of their distinctive clinical, radiographic, histologic, immunohistochemical, and molecular features. These cases are frequently submitted for consultation, further suggesting the diagnostic difficulties inherent to these tumors. Specifically, olfactory neuroblastoma is a neoplasm that can histologically mimic many tumors within the sinonasal tract, making recognition of this tumor important, as the management frequently requires a bicranial-facial surgical approach, a trephination procedure which can be quite technically difficult and challenging to achieve a good result. The management is therefore quite unique in comparison to other sinonasal tract malignancies, setting it apart diagnostically and managerially from other lesions.     

Amyloidosis and multiple myeloma. A reevaluation using a control population

To explore the possible influence of multiple myeloma on age-related amyloid accumulation, the frequency, extent and distribution of amyloid deposition in 35 patients who died of multiple myeloma was compared to that observed in 138 patients of comparable age but without myeloma (controls). The presence of multiple myeloma did not appear to enhance the development of age-related amyloid.Amyloid deposition in the patients with myeloma could be distinguished from that in the patients without myeloma by the occasional finding of amyloid in the bone marrow. In addition, material having the tinctorial and ultrastructural characteristics of amyloid was found in the renal tubular casts of 15 patients with myeloma but in none of the controls.The results suggest that although the appearance of amyloid in certain locations appears to be characteristic of multiple myeloma, the presence of amyloid in the heart or vascular system, even in considerable amounts, in a patient with multiple myeloma does not necessarily imply that the two conditions are related.