脱细胞基质面神经修复材料的制备

目的 制备具有天然神经组织结构的支架,构建组织工程化面神经用于修复面神经损伤。方法 取家兔面神经,改良化学萃取法制备脱细胞神经基质,HE染色形态学观察去细胞及脱髓鞘情况,荧光分光光度计测定支架内细胞经Quant-iT PicoGreen工作液染色后的DNA含量。MTT法检测细胞在支架上的相对生长率从而检测支架的细胞毒性。结果 支架移植体呈圆柱形,弹性与正常神经基本一致,组织观察显示细胞结构未见残余完整细胞及细胞碎片残留,未见神经髓鞘及轴突结构,细胞外基质形成纵向排列结构,结构之间可见空隙。兔脱细胞面神经基质支架内残留的DNA含量较正常兔面神经明显下降(P<0.01)。神经基质供体无细胞毒性。结论 改良化学萃取法可有效去除面神经细胞,天然结构保存完好,细胞毒性低,可作为组织工程化面神经的支架。     

microRNAs及miR-214在神经损伤再生中的作用

microRNAs(miRs)是一类短的非编码RNA基因,参与了神经系统的生理和病理过程,许多miRs在获得性脑损伤、脊髓损伤和周围神经损伤等均存在差异表达,这些miRs主要通过靶向特异性mRNA来影响神经存活、神经再生和神经胶质表型,从而导致miRs的翻译抑制或mRNA降解。周围神经损伤会使患者躯干和四肢出现神经功能障碍,严重影响患者的劳动力和生活质量。因此,研究miRs在神经损伤和再生中的调控作用,可为治疗神经系统疾病找到潜在的治疗靶点,对拓展神经系统疾病治疗的策略具有重要的意义。microRNA-214(miR-214)是miRs家族的成员之一,有研究显miR-214在神经元分化中被调节,并且在体外控制神经突生长。本文就miRs在神经损伤中的研究进展和miR-214在周围神经再生过程中可能的作用作一综述。     

神经再生相关营养因子的提取及生物学特性检测

目的 提取神经再生微环境中的神经再生营养活性物质.方法 取York猪暴露坐骨神经,截断后缝接入硅胶管中,形成神经再生室,吸取硅胶管内液体,凝胶柱层析分离纯化神经再生液,雪旺细胞(SC)增殖实验检测所提取的神经再生营养因子的活性.结果 凝胶层析分离神经再生室内液体可得到2个峰,经检测2号蛋白峰可以促进SC增殖.结论 神经再生液中的提取物具有神经营养功能,可用于周围神经修复.     

同种异体复合组织移植后神经再生的研究进展

同种异体复合组织移植是修复周围组织严重缺损的理想选择,而移植组织的功能包括感觉和运动功能,都依赖于移植神经的再生和功能恢复。本文就各种复合组织异体移植后移植神经的再生机制、动物试验和临床上神经再生状况以及免疫治疗方面的研究进展作一综述。     

Adipose derived stem cells and nerve regeneration

Injuries to peripheral nerves are common and cause life-changing problems for patients alongside high social and health care costs for society. Current clinical treatment of peripheral nerve injuries predominantly relies on sacrificing a section of nerve from elsewhere in the body to provide a graft at the injury site. Much work has been done to develop a bioengineered nerve graft, precluding sacrifice of a functional nerve. Stem cells are prime candidates as accelerators of regeneration in these nerve grafts. This review examines the potential of adipose-derived stem cells to improve nerve repair assisted by bioengineered nerve grafts.     

Instructions to Authors简

<正>Aims and Scopes Neural Regeneration Research(NRR;ISSN 1673-5374)is an open-access,peer-reviewed only international journal focusing exclusively on the exciting field of neural regeneration.NRR is devoted to publishing basic research,translational medicine and randomized clinical trial articles,as well as prospective reviews written by invited experts in the field of neural regeneration.NRR publishes a diverse array     

Letter from Editors-in-Chief

<正>Dear readers and contributors,Welcome to the first issue of Neural Regeneration Research(NRR)in 2014.Over the past few years,NRR has dedicated its focus to the rapidly growing field of neural regeneration.The editorial team has made significant contributions to soliciting higher quality papers and many efforts,described below,have been or will be made to make NRR one of the leading journals in the field of neural regeneration.     

Puerarin accelerates neural regeneration after sciatic nerve injury

Puerarin is a natural isoflavone isolated from plants of the genus Pueraria and functions as a protector against cerebral ischemia. We hypothesized that puerarin can be involved in the repair of peripheral nerve injuries. To test this hypothesis, doses of 10, 5, or 2.5 mg/kg per day puerarin(8-(β-D-Glucopyranosyl-7-hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) were injected intraperitoneally into mouse models of sciatic nerve injury. Puerarin at the middle and high doses significantly up-regulated the expression of growth-associated protein 43 in the L4–6 segments of the spinal cord from mice at 1, 2, and 4 weeks after modeling, and reduced the atrophy of the triceps surae on the affected side and promoted the regeneration of nerve fibers of the damaged spinal cord at 8 weeks after injury. We conclude that puerarin exerts an ongoing role to activate growth-associated protein 43 in the corresponding segment of the spinal cord after sciatic nerve injury, thus contributing to neural regeneration after sciatic nerve injuries.