系统性红斑狼疮女性患者血清CA125,CA199化学发光法测定的临床应用

目的 :探讨系统性红斑狼疮 (SLE)女性患者血清肿瘤标志物CA12 5 ,CA199含量变化及临床意义。方法 :用化学发光法测定 30例正常女性和 38例SLE女性患者血清中CA12 5 ,CA199含量。结果 :正常组CA12 5含量为 11.14± 6 .4 8U/ml,CA199含量为 3.75± 2 .89U/ml;SLE组CA12 5 ,CA199分别为 2 2 .5 6± 2 0 .4 6U/ml,9.5 7± 9.34U/ml。SLE患者CA12 5 ,CA199阳性率分别为 2 1.1% ,7.89%。结论 :SLE患者血清CAl2 5 ,CA199含量较正常组增高 (P <0 .0 5 )。CA12 5 ,CA199在SLE女性患者中可出现阳性 ,对临床诊断SLE有一定价值。     

Urinary 5-Hydroxytryptophol: A Possible Marker of Recent Alcohol Consumption

Urinary 5-hydroxytryptophol (5-HTOL) is currently being evaluated as a marker of recent alcohol consumption. To compensate for urinary dilution, the molar ratio between 5-HTOL and 5-hydroxyindole-3-acetic acid (5-HIAA) is used. The 5-HTOL/5-HIAA ratio showed a satisfactory degree of individual stability when it was followed in a group of teetotallers for 1 month. The mean value of 5-HTOL/5-HIAA in a group of 69 persons abstaining from alcohol was 7.6 (pmoles 5-HTOL/nmoles 5-HIAA). Ninety-seven percent had values ranging from 4 to 17, with no value exceeding 20. A group of healthy volunteers were tested 12 hr after alcohol consumption and showed a dose-dependent and statistically significant elevation in the 5-HTOL/5-HIAA ratio. Four regular alcohol consumers who were followed during a period of 3 months of drinking had elevated values of the 5-HTOL/5-HIAA ratio in 60% of their urine samples. The present study indicates that urinary 5-HTOL/5-HIAA is a sensitive and reliable marker of recent alcohol consumption. We propose that a 5-HTOL/5-HIAA ratio greater than 20 (pmoles/nmoles) can be used to indicate recent alcohol consumption. This limit gives a low frequency of false positives; the statistical probability of having a value greater than 20 during abstinence from alcohol was calculated to be less than 0.001.     

乙肝病毒标志物阳性肺结核患者抗结核治疗肝功能损害的观察

目的 :观察抗结核药物对HBV M阳性肺结核患者肝功能的影响。方法 :比较HBV M阳性和阴性肺结核患者抗结核治疗肝功能损害的情况。结果 :HBV M阳性患者肝损率比阴性者明显增高。结论 :抗结核药物致肝损害 ,HBV M阳性者比阴性者多 ,可能与用药前肝脏病理损害严重有关。应选用肝损害小的药物并积极采取综合措施进行防范     

大肠癌临床分期相关蛋白的蛋白质组学研究

目的：筛选与大肠癌临床分期相关的蛋白，为大肠癌分子分期和预后预测提供依据。方法：将不同临床分期大肠癌组织蛋白进行二维凝胶电泳，选择部分差异表达蛋白进行MALDI-TOF质谱分析和生物信息学分析以鉴定差异表达蛋白;免疫组织化学方法验证筛选结果。结果： 建立了不同临床分期大肠癌组织的二维凝胶电泳图谱，其中Ⅰ，Ⅱ，Ⅲ，Ⅳ大肠癌组织平均蛋白质点数分别为970±41,980±32,1010±43,1240±34;以Ⅰ期大肠癌为参照，Ⅱ期大肠癌差异表达蛋白有52.00±12，Ⅲ期大肠癌差异表达蛋白42.00±11，Ⅳ期大肠癌差异表达蛋白72.00±15，通过进行质谱分析和生物信息学查询，鉴定30个显著差异表达的蛋白点，其中Ⅱ，Ⅲ，Ⅳ均上调的有3种蛋白：AnnexinⅡ,AnnexinⅣ,热休克蛋白27(HSP27)。而仅在Ⅳ期中上调蛋白有1种蛋白,即肝脂肪酸结合蛋白（LFABP)。AnnexinⅡ和肝型脂肪酸结合蛋白表达的免疫组化检测结果与蛋白质筛选结果基本一致。结论：不同临床分期的大肠癌中存在着差异表达蛋白，这些蛋白可能作为大肠癌分子分期和预后的标志物。     

蛋白芯片检测系统(多肿瘤标志物C12系统)的应用评价

目的：应用并评价蛋白芯片检测系统(多肿瘤标志物C12)的临床价值。方法：应用雅培电化学发光系统(ECL)和C12系统双盲法检测41份血清标本。结果：在对照检测达10份标本的AFP、CEA、β-HCG三个项目，C12系统和ECL系统符合性达81．8％以上；C12系统多个指标间可相互支持其准确性，并有助于判断肿瘤来源。结论：C12系统具有较高准确性，其指标间可相互支持，并有助于判断肿瘤源性。     

丙型肝炎病毒标志物测定的临床应用

目的 :探讨丙型肝炎病毒 (HCV)感染者血清及初乳中该病毒标志物存在状况。　方法 :对 2 44份 HCV感染者、33份对照血清和 35份 HCV特异性 Ig G抗体阳性的产妇、41份健康产妇的初乳进行 HCV四种标志物测定。抗HCV- Ig G、Ig M和 GOR抗体采用间接 EL ISA法 ,HCV C33抗原采用双抗体夹心 EL ISA法。　结果 :抗 HCV- Ig G、抗 HCV- Ig M、HCV C33和抗 GOR抗体四种标志物在慢性活动性丙型肝炎组检出率最高 ,分别为 10 0 .0 %、85 .7%、46 .4%和 6 4.3% ;急性输血后丙型肝炎组检出率为 6 6 .7%、10 0 .0 %、2 2 .9%和 42 .9% ;慢性稳定性丙型肝炎组为 94.1%、2 6 .5 %、17.6 %和 5 2 .9% ;血透患者组为 6 2 .6 %、31.3%、4.8%和 45 .6 %。血清抗 HCV Ig G阳性组初乳为 17.1%、2 .9%、8.5 %和 37.1%。血清和初乳对照组除 1例献血员血清抗 HCV- Ig G阳性外 ,其余均为阴性结果。　结论 :除抗 HCV- Ig G外 ,抗 HCV- Ig M、HCV C33和 GOR抗体三种标志物在 HCV感染的相关疾病组中 ,有一定的阳性检出率 ,均可作为 HCV感染的标志 ,可用于 HCV感染后的实验室诊断。     

Release of intracellular enzymes from cutaneous cells after non-necrotizing damage by ultraviolet light

Summary Experimental blisters were produced with suction on normal human skin and simultaneously on skin inflamed after exposure to middle wave ultraviolet light. Total proteins and marker enzymes for the plasma membrane, cytosol, lysosomes, peroxisomes, mitochondria, and microsomes were assayed in the blister fluid. In blisters on erythematous skin, a large increase of lactate dehydrogenase from cytosol was noted. A small increase of the plasma membrane marker phosphodiesterase I and some increase of -mannosidase from lysosomes was also found. No significant increase in total proteins or in the markers for peroxisomes was observed, whereas mitochondrial and microsomal marker enzymes were not detectable.It is concluded that cutaneous cells to some extent may lose intracellular enzymes without visible signs of irreversible damage (necrosis), but that an UVB-induced injury/regeneration cycle probably explains the enzyme release.     

Differences in the response of Sprague-Dawley and Lewis rats to bezafibrate: The hypolipidemic effect and the induction of peroxisomal enzymes

The effects of bezafibrate administered at 10 and 50 mg/kg/day for 7 days to male Sprague-Dawley (SD) and Lewis rats were investigated in order to determine the interrelation between the changes in serum and hepatic lipid contents and activities of selected peroxisomal, microsomal and mitochondrial enzymes in the two rat strains. In both strains, bezafibrate effectively reduced serum and hepatic lipids, increased the liver weight, induced a proliferation of peroxisomes, and selectively elevated the activities of carnitine acetyltransferase and of the enzymes of the peroxisomal -oxidation system. Moreover, immunoblotting revealed that the drug specifically enhanced the concentration of only those peroxisomal enzymes involved in fatty acid -oxidation. The data obtained demonstrate that although the responses initiated by bezafibrate are qualitatively similar in both strains, they differ in their magnitude in a dose-dependent manner, with the Lewis strain exhibiting a more pronounced response than the SD rats. These results show that dose-dependent strain differences as well as the generally known species differences should be taken into account in pharmacological and toxicological evaluations of fibrates in rodents. Furthermore, generalization and extrapolation from rodent studies should be treated with great caution.     

急性白血病患者血清bFGF与TSGF水平的研究

目的探讨急性白血病(AL)患者血清碱性成纤维细胞生长因子(bFGF)及恶性肿瘤相关物质群(TSGF)的水平及其临床意义。方法应用酶联免疫法(ELISA)及化学显色法分别对47例AL初治(AL初治组)、8例AL复发(AL复发组)患者化疗前后血清bFGF及TSGF的水平进行检测,并与正常人对照组比较;47例AL初治患者化疗后,根据疗效观察,其中11例CR(完全缓解组,CR组)、13例NR(未缓解组,NR组),对CR组与NR组患者血清bFGF及TSGF的水平进行对比分析。结果AL初治组及AL复发组血清bFGF水平均明显高于正常人对照组水平,有非常显著性差异(t=6.71、6.18,P均<0.001);AL初治组及AL复发组血清TSGF水平均明显高于正常人对照组水平,差异非常显著(t=6.847、5.009,P均<0.001);CR组血清bFGF水平及TSGF水平均明显下降,与正常人对照组相比较,均无显著性差异(t=1.11、1.482,P均>0.05);ALLNR组化疗前血清bFGF水平明显高于ALLCR组化疗前的水平,具有显著性差异(t=2.43,P<0.05),AMLNR组与AMLCR组化疗前血清bFGF水平无显著性差异(t=0.76,P>0.05);ALL与AMLNR组化疗前血清TSGF水平均高于CR组化疗前的水平,且均具有显著性差异(t=3.29,2.13;P<0.01,0.05);血清bFGF水平与TSGF水平呈正相关(γ=0.5263,P<0.01)。结论联合检测血清bFGF及TSGF水平对了解AL的发生、发展     

Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole ( Microtus agrestis,Rodentia)

Cell proliferation in developing tooth germs has been studied particularly using bromodeoxyuridine (BrdU) incorporation into growing tooth primordia and by counting and three-dimensional (3D) reconstruction of mitoses in serial sections of developing teeth. PCNA has been proposed as an alternative marker of proliferation activity. The aim of our study was to detect immunohistochemically locations of PCNA-positive cells in developing tooth germs of Microtus agrestis (Rodentia). PCNA expression could be distinguished in oral epithelium and mesenchyme before first signs of dental lamina elevation. During bud, cap, and bell stages, positive immunostaining could be observed at defined sites in enamel organ, tooth papilla, and dental follicle. Rudimental tooth germs of the upper diastema, enamel knots, and inner enamel epithelium at day of ontogeny 18 and 19 showed negative reaction. PCNA marks cycling and early G0 cells and can be used successfully as a proliferation marker even in collection material.