Burkholderia thailandensis E264/pBMTL3-tdpR发酵生产抗癌药物ThailandepsinA的研究

在伯克氏菌Bth264野生株产新型抗癌药物Thailandepsin A以及调节基因tdp R正向调控Thailandepsin A生物合成的基础上,利用基因工程菌Bth264/p BMTL3-tdp R发酵生产Thailandepsin A,以提高产量。以0.5%乳糖为诱导剂,确定最佳诱导条件:发酵15 h添加乳糖,诱导时间6 h;通过单因素实验,确定葡萄糖和胰蛋白胨作为碳氮源、装液量65/250 m L以及接种量1%;同时结合优化发酵培养基进行发酵,Thailandepsin A产量达到252.14 mg·L-1,比优化前的产量提高56%;另外在发酵过程中,添加大孔树脂HP-20原位吸附产物,Thailandepsin A产量可达283.75 mg·L-1,比不加树脂提高13.8%;最后,基于RT-PCR和比较Ct值法,基因工程菌和野生菌相比,Thailandepsin A生物合成基因tdp B、tdp C1的转录水平分别提高11.4倍和6.0倍,对应的产量增加4.6倍,从而在很大程度上说明调节基因tdp R的过表达促进生物合成基因转录水平的提高以及产量的增加。     

一种基于H.264 Intra帧的差错隐藏方法

为了充分利用H.264新特性带来的冗余信息对视频传输中的错误进行隐藏,提出了一种新的基于Intra帧的差错隐藏方法,根据丢失块周围子块的预测模式推断出丢失块中各子块的预测模式,再根据推断的预测模式重建丢失块. 试验表明,这种方法能很好地预测出丢失块的预测模式,并能适应不同内容类型的图像,差错隐藏的效果明显.     

基于代价策略的H.264/AVC半脆弱水印算法

In order to solve some existing problems on detecting malicious tampering by video watermarking based on H.264/AVC video compression standard,a semi-fragile video watermarking algorithm for H.264/AVC based on cost strategy was proposed.To further improve the robustness of the feature codes in semi-fragile video watermarking algorithm,a new generation algorithm for feature codes by eliminating the correlation of them was proposed.In order to meet the requirement that the semi-fragile watermarks should be uniformly embedded into the video frames to locate the tampered region accurately,a minimal cost function was introduced to select a DCT coefficient in the 4 × 4 block residual to embed watermark which had minimal impact on the video quality and bit rates.Experimental results show that the proposed algorithm can significantly reduce impact on video quality and bit rate because of embedding the watermarking by adopting the minimum cost function and it is sensitive to malicious manipulations.The retrieved feature code and watermarking can detect and locate malicious manipulations.     

基于改进KNN算法的AVS到H.264/AVC 快速转码方法

尽管音视频编码标准(Audio and Video Coding Standard,AVS)的编码性能可以与H.264相媲美,但是H.264的应用范围更加广泛,因此视频由AVS标准转码成H.264标准具有很大的应用前景.目前,主流的转码方法是将AVS的分块模式与H.264的分块模式映射的方式降低转码复杂度,但是技术之间的差异导致这两种标准之间的分块模式并不是一一映射的关系,因此会导致编码效率大幅度降低.提出一种基于改进KNN(K最邻近节点)算法的AVS到H.264/AVC快速转码方法.充分利用了AVS码流中的各种信息,通过改进的KNN算法建立了中间信息和H.264分块模式之间的映射模型.根据AVS中运动矢量信息的差异自适应确定H.264可能的分块模式,实验结果表明上述问题得到有效解决,该算法在保证H.264编码效率的前提下大幅降低了转码复杂度.     

基于网络和H.264的视频监控系统设计

设计了一种使用网络和H.264视频编解码的监控系统实施方案。方案采用TI公司的DM6437 DSP作为硬件编码模块,对摄像头采集的视频进行H.264编码,编码后的视频数据借助RTP实时传输协议和UDP套接字在网络传输,并最终在PC机上实现实时视频的接收和显示。测试结果表明,该方案实现方便、效果良好,具有潜在的应用价值。     

3G移动无线视频监控技术在煤矿的应用

介绍了视频编码H.264标准和3G网络连接技术,针对矿井复杂的工作环境,提出了利用3G网络技术实现无线视频监控的方法,选择适用于3G环境背景的H.264编码标准以及信道传输RTP/RTCP协议,实现了移动远程视频监控矿井内部生产状况的目的。     

地方视频会议会商系统的建设与应用

建设多媒体业务的视频会议系统,综合语音、图像、数据等信息,通过网络介质进行远距离高速传输,可增强在不同区域交流的亲切感和临场感,提供一种简便而有效的工作手段,充分发挥视频会议实时高效的特点,将显著提高决策和工作效率,尤其能为政府在防灾减灾的指挥决策方面起到积极快速反应的作用。     

Efficient disparity vector prediction schemes with modified P frame for 2D camera arrays

An efficient disparity estimation algorithm for multi-view video sequences, recorded by a two-dimensional camera array in which the cameras are spaced equidistantly, is presented. Because of the strong geometrical relationship among views, the disparity vectors of a certain view can for most blocks be derived from the disparity vectors of other views. A frame constructed using that idea is called a D frame in this work. Three new prediction schemes which contain D frames are proposed for encoding 5 × 3 multi-view video sequences. The schemes are applied to several multi-view image sequences taken from a camera-array and they are compared in terms of quality, bit-rate and complexity. The experimental results show that the proposed prediction schemes significantly decrease the complexity of the encoder at a very low cost of quality and/or bit-rate.     

A real-time dual watermarking algorithm of H.264/AVC video stream for Video-on-Demand service

A real-time dual watermarking algorithm of H.264/AVC compressed video is proposed for Video-on-Demand (VOD) service. The copyright information and user information are modulated by CDMA spreading strategies as watermark. At the encoder side, copyright information is embedded into the first non-zero coefficient of Intra 4 × 4 coded blocks in the luminance components of I frames. An effective error compensation mechanism is simultaneously introduced into the embedding process, which strictly restricts the distortion caused by quantization and watermark embedding within a step size of quantization. At the server side, a XOR based mapping rule is utilized for watermark embedding because the number of non-zero coefficients after quantization is quite less in P frames. Every two bits of the private user information is mapped to three non-zero coefficients of P frames. Experimental results on test sequences demonstrate that the proposed approach has just slight influence on bit rate and PSNR with real-time performance for VOD services, and achieves the dual purposes of copyright protection and pirate tracking.     

一种基于YHFT-Matrix DSP的去块效应滤波算法的向量化实现

针对H.264视频压缩编码标准中去块效应滤波器部分提出了一种基于YHFT Matrix DSP的并行设计及向量实现方法。重点对H.264协议中去块效应滤波器进行理论分析，并利用向量数据访问单元、向量处理单元、高效的混洗单元和灵活的矩阵对其进行并行算法设计。将去块滤波算法分别映射到YHFT Matrix和TI的TMS320C6415中，通过统计两者性能，表明YHFT Matrix的性能优于TMS320C6415。