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1.
为推进病历数字化发展,并确保其信息的安全性,将以HIS电子病历系统为基础,采用安信数字签名技术和PKI或PMI系统搭建相信并加以任用的授权服务,经过针对实际的PKC和CA的确认、委托与管控构建整体的数字签名平台,完成电子病历数字签名功能设计,以加强电子文件的完整性、真实性和不可抵赖性。最后以某医院的XML结构化的电子病历系统为基础进行项目实施,完成了医护人员通过HIS的快速身份认证,和准确地数字签名。 相似文献
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Gianmarco Mangiaterra Elisa Carotti Salvatore Vaiasicca Nicholas Cedraro Barbara Citterio Anna La Teana Francesca Biavasco 《International journal of molecular sciences》2021,22(4)
The occurrence of Pseudomonas aeruginosa (PA) persisters, including viable but non-culturable (VBNC) forms, subpopulations of tolerant cells that can survive high antibiotic doses, is the main reason for PA lung infections failed eradication and recurrence in Cystic Fibrosis (CF) patients, subjected to life-long, cyclic antibiotic treatments. In this paper, we investigated the role of subinhibitory concentrations of different anti-pseudomonas antibiotics in the maintenance of persistent (including VBNC) PA cells in in vitro biofilms. Persisters were firstly selected by exposure to high doses of antibiotics and their abundance over time evaluated, using a combination of cultural, qPCR and flow cytometry assays. Two engineered GFP-producing PA strains were used. The obtained results demonstrated a major involvement of tobramycin and bacterial cell wall-targeting antibiotics in the resilience to starvation of VBNC forms, while the presence of ciprofloxacin and ceftazidime/avibactam lead to their complete loss. Moreover, a positive correlation between tobramycin exposure, biofilm production and c-di-GMP levels was observed. The presented data could allow a deeper understanding of bacterial population dynamics during the treatment of recurrent PA infections and provide a reliable evaluation of the real efficacy of the antibiotic treatments against the bacterial population within the CF lung. 相似文献
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Analysis of gas holdup and bubble behavior in a biopolymer solution inside a bioreactor using tomography and dynamic gas disengagement techniques 下载免费PDF全文
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摘要:为了探究不同镁含量对非调质钢中组织和硫化物形态、尺寸、分布及成分的影响,采用蔡司金相显微镜、扫描电子显微镜、小样电解等方法,分析了经高温电阻炉冶炼不同镁添加量的49MnVS3非调质钢。结果表明,由于镁蒸气压较高,在实验室冶炼中大量挥发,导致钢中镁的实际平均收得率仅为310%;镁的质量分数为0~22×10-6时,随着镁的质量分数的增加,钢中硫化物形态由Ⅱ类逐渐向Ⅰ类、Ⅲ类转变;钢中硫化物尺寸增大,硫化物的分布均匀性得到显著改善;钢中复合夹杂物比例明显增加,但MnS的比例出现下降;形成了细小弥散的氧化物,增加了奥氏体形核质点,具有细化组织的趋势。 相似文献
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Cellulose dissolved in ionic liquid (1‐(carboxymethyl)pyridinium chloride)/water (60/40 w/w) mixture is regenerated in various non‐solvents, namely water, ethanol, methanol and acetone, to gain more insight into the contribution of non‐solvent medium to the morphology of regenerated cellulose. To this end, the initial and regenerated celluloses were characterized with respect to crystallinity, thermal stability, chemical structure and surface morphology using wide‐angle X‐ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy and scanning electron microscopy. According to the results, regardless of non‐solvent type, all regenerated samples have the same chemical structure and lower crystallinity in comparison to the initial cellulose, making them a promising candidate for efficient biofuel production based on enzymatic hydrolysis of cellulose. The reduction in crystallinity of regenerated samples is explained based on the potential of the non‐solvent to break the hydrogen bonds between cellulose chains and ionic liquid molecules as well as the affinity of water and non‐solvent which can be evaluated based on Hansen solubility parameter. The latter also determines the phase‐separation mechanism during the regeneration process, which in turn affects surface morphology of the regenerated cellulose. The pivotal effect of regenerated cellulose crystallinity on its thermal stability is also demonstrated. Regenerated cellulose with lower crystallinity is more susceptible to molecular rearrangement during heating and hence exhibits enhanced thermal stability. © 2019 Society of Chemical Industry 相似文献
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Yasoub Eghbali Mahmoud Ferdosizade Naeiny 《International Journal of Communication Systems》2020,33(14)
In this paper, an off‐grid direction of arrival (DoA) estimation method is proposed for wideband signals. This method is based on the sparse representation (SR) of the array covariance matrix. Similar to the time domain DoA estimation methods, the correlation function of the sources was assumed to be the same and known. A new measurement vector is obtained using the lower‐left triangular elements of the covariance matrix. The DoAs are estimated by quantizing the entire range of continuous angle space into discrete grid points. However, the exact DoAs may be located between two grid points; therefore, this estimation has errors. The accuracy of DoA estimation is improved by the minimization of the difference between the new measurement vector and its estimated values. Simulation results revealed that the proposed method can enhance the DoA estimation accuracy of wideband signals. 相似文献
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In this study, the impacts of non‐integer order controller on the stable parameter space of the microgrid (MG) frequency control system with fixed communication time delay are investigated and discussed with the help of the stability boundary locus (SBL) method. This study proposes a non‐integer order controller for the load frequency control (LFC) of the MG systems. To that end, the load frequency model of the MG is formulated and then the characteristic equations of this model are obtained. Then, with the help of this characteristic equation, the stable parameter space of the non‐integer controller is determined with regard to different time delay (τ ) and fractional integral order values (α ) using the SBL method. In order to show the accuracy of the obtained stable parameter space, time domain and generalized modified Mikhailov (GMM) criterion studies are carried out for different values of (τ ) and (α ). According to the results obtained, the areas of stable parameter space according to different α values and τ = 1.6 are calculated as 444.8860 for α = 0.4 , 342.9728 for α = 0.7 , 259.3578 for α = 1 , 216.2541 for α = 1.3 and 159.6826 for α = 1.6 . In addition, the areas of stable parameter space according to different τ values and α = 1.4 are calculated as 784.5222 for τ = 1 , 106.3219 for τ = 2 , 29.6959 for τ = 3 and 11.5946 for τ = 4 . Despite the extreme variability arising from nature of resources that make up the MG, the designed non integer order controller with the values selected within the stable parameter space stably carries out LFC control of the MG. 相似文献
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