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1.
番茄与黄瓜远缘嫁接愈合口形成过程的组织解剖分析 总被引:1,自引:0,他引:1
为探明番茄/黄瓜远缘嫁接不亲和时愈合口的形成过程,为后期砧穗间信息互作的研究奠定一定的理论基础,以黄瓜‘新泰密刺’、黑籽南瓜和番茄‘中杂9号’为试材,采用下胚轴斜靠接方法远缘嫁接番茄/黄瓜(接穗/砧木),嫁接组合黄瓜/南瓜、黄瓜/黄瓜作为嫁接亲和性的对照。观察嫁接后第2、5、8和15天嫁接苗生长形态变化并统计成活率,对比分析黄瓜的近缘及远缘嫁接的嫁接口愈合过程。结果表明:1)嫁接接口处石蜡切片结果显示,对照黄瓜自嫁接和黄瓜与南瓜的嫁接均经历了相同的愈合过程,并最终成功地建立了形成层与输导组织,得以正常生长;2)分别用经木质部运输的品红溶液、经韧皮部运输的HPTS染料标记嫁接苗,结果显示远缘的番茄与黄瓜嫁接后,砧木与接穗虽然可以各自分化出愈伤组织,但二者愈伤组织的薄壁细胞无法真正地融合,机械性地对接在一起的木质部可以运输水分,使得番茄接穗不萎蔫,但韧皮部并未连通,无法进行其光合及同化产物运输、维持其生命活动,初步解释了远缘嫁接不亲和的解剖学过程。 相似文献
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There are several skin grafting methods described in the human and animal literature. Currently, there are five types of free grafts used in horses: pinch and punch grafts, split and full-thickness sheet or mesh grafts and tunnel grafts. Published methods of tunnel grafting describe the use of alligator forceps. The alligator forceps create a poor tunnel and are excessively traumatic to the granulation bed. This technique utilised a 13G Jamshidi needle that was placed across the granulation bed and created a uniform tunnel. The Jamshidi needle was atraumatic to the granulation bed increasing the opportunity for graft survival. A twin bladed scalpel allowed for the quick creation of uniform width grafts. Removal of the overlying tunnel ‘roof’ took place 5–14 days later to allow graft expansion. This case series included five horses with distal limb wounds and one with a wither injury. Four horses required general anaesthesia for graft placement and three required general anaesthesia for the removal of the tunnel roof. The acceptance of the grafts varied from 70% to 100%. Graft expansion to cover the granulation tissue took 2–5 months. This case series demonstrates that this technique of graft production and placement is an easy method for achieving successful skin grafting. Compared to other graft types, tunnel grafts are more readily accepted. Cosmetic and functional results achieved are better than those with pinch and punch grafts. Tunnel grafting does not require expensive equipment or advanced training, and in some cases can be performed under standing sedation. 相似文献
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Masaaki Katayama Yasuhiko Okamura Shunsuke Shimamura Rieko Katayama Hiroaki Kamishina Yuji Uzuka 《Research in veterinary science》2014
Graft perfusion with cold heparinized saline has known to induce ischemia and reperfusion injury in feline kidney transplantation. In this study, the effects of phosphate-buffered sucrose solution and heparinized saline solution on early kidney graft function were compared in feline kidney autotransplantation. Perfusion of grafts with or without hypothermic storage with chilled phosphate-buffered sucrose solution prevented ischemia and reperfusion injury despite a very short ischemic time. The results of our study suggest that phosphate-buffered sucrose perfusion and storage solution should be effective to reduce ischemia and reperfusion injury despite a very short ischemic time in feline kidney transplantation. 相似文献
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TAN Shu-fang ZHANG Li-zhi HUANG Lin-jie CHEN Ming ZHANG Wei SHI Jian-ting TANG Tian-tian LIN Xiao-ling 《园艺学报》2018,34(8):1383-1389
AIM:To investigate the relationship between blood eosinophil count and risk of postoperative pneumonia in patients with coronary artery bypass graft (CABG) surgery. METHODS: The medical records of 613 patients who underwent CABG surgery from January 2008 to December 2017 in our hospital were collected and reviewed. The incidence of postoperative pneumonia and hospital mortality were compared in the patients with low or high blood eosinophil count. The risk factors for postoperative pneumonia following CABG surgery were analyzed by multiple logistic regression. RESULTS: The patients enrolled in the study were 582, in which 220 patients had preoperative blood eosinophil counts of less than 2% (low blood eosinophil count) and 362 patients had eosinophil counts of 2% or more (high blood eosinophil count). The incidence of postoperative pneumonia in the patients with low blood eosinophil count was 14.1% (31/220), significantly higher than that (6.4%, 23/362) in the patients with high blood eosinophil count (P=0.002). However, the hospital mortality of the patients with high or low blood eosinophil count had no difference. Low blood eosinophil count (OR=3.521, 95% CI:1.213~10.223, P=0.021), nasogastric tube (OR=6.490, 95% CI:2.757~15.280, P<0.001) and mechanical ventilation ≥ 24 h (OR=3.496, 95% CI:1.156~10.178, P=0.035) were identified as independent risk factors for postoperative pneumonia following CABG surgery. CONCLUSION: Low blood eosinophil count is associated with increased risk of postoperative pneumonia following CABG surgery. 相似文献
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卡里佐枳橙是重要的柑橘砧木,在世界范围内广泛应用。20世纪90年代作为一种新砧木被引进我国并在部分地区用于柑橘生产。为了全面了解卡里佐枳橙在我国的生产表现,本研究采用田间观察和问询相结合的方法在全国6个柑橘主产省16个区(县)30多个果园开展了嫁接亲和性、早结丰产性、抗逆性等方面的调查。在重庆和广西两个不同的生态类型共三个试验点、分别对嫁接在卡里佐枳橙和枳砧上的梁平柚和纽荷尔脐橙等两个不同接穗品种开展了连续三年在树体、产量、结果效率和果实品质等园艺学性状方面的系统比较。结果显示,总体上讲卡里佐枳橙的嫁接亲和性较好,但与红江橙和无籽柠檬嫁接后表现出明显的不亲和;早结丰产性好,树势旺,结果效率不如枳砧高;果实品质优良,与枳砧相比无显著性差异。在抗逆性方面,卡里佐枳橙对裂皮病、天牛比较敏感,对碱性土壤比较敏感,容易导致叶片缺铁黄化。本研究将对我国柑橘砧木的选择以及以卡里佐枳橙砧果园的田间管理提供参考和指导。 相似文献
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通过对涝渍胁迫条件下豫楸1号4种砧木嫁接苗渗透调节物质(Pro、可溶性糖、可溶性蛋白)活性的测定,结果表明:在持续涝渍胁迫下,3种渗透调节物质的变化趋势基本一致,整体变化趋势为先上升后下降,只是峰值出现的时间有差异。 相似文献
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