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Manuel Muñoz Esther Julián Merçé Garcia-Barceló Vicente Ausina Marina Luquin 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1997,689(2):133
The mycolate pattern of a recently recognized mycobacterial pathogen, Mycobacterium mucogenicum (formerly Mycobacterium chelonae-like organism), was established for the first time. The reference strains, together with 31 environmental and clinical isolates belonging to this species, were examined for their mycolate composition by thin-layer chromatography. All strains tested exhibited the same mycolate profile. Mycolates were identified as belonging to the type without additional oxygenated chemical groups (mycolate I) and the type with a dicarboxylic group (mycolate VI); the identification of the latter was reinforced by the presence of 2-octadecanol, as seen by gas-liquid capillary chromatography. This mycolate profile permits the clear differentiation of M. mucogenicum from other related species, as members of the Mycobacterium fortuitum complex. This fact is especially important because strains of M. mucogenicum are very difficult to differentiate from other species of the M. fortuitum complex by means of conventional biochemical tests. Moreover, the characteristic mycolate profile exhibited by the strains of M. mucogenicum supports the recent proposal which considers them as members of a new species. 相似文献
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Yong Yang Alexandra Bhatti Danxia Ke Mercedes Gonzalez-Juarrero Anne Lenaerts Laurent Kremer Yann Guerardel Peijun Zhang Anil K. Ojha 《The Journal of biological chemistry》2013,288(1):382-392
Mycobacteria are shaped by a thick envelope made of an array of uniquely structured lipids and polysaccharides. However, the spatial organization of these molecules remains unclear. Here, we show that exposure to an esterase from Mycobacterium smegmatis (Msmeg_1529), hydrolyzing the ester linkage of trehalose dimycolate in vitro, triggers rapid and efficient lysis of Mycobacterium tuberculosis, Mycobacterium bovis BCG, and Mycobacterium marinum. Exposure to the esterase immediately releases free mycolic acids, while concomitantly depleting trehalose mycolates. Moreover, lysis could be competitively inhibited by an excess of purified trehalose dimycolate and was abolished by a S124A mutation affecting the catalytic activity of the esterase. These findings are consistent with an indispensable structural role of trehalose mycolates in the architectural design of the exposed surface of the mycobacterial envelope. Importantly, we also demonstrate that the esterase-mediated rapid lysis of M. tuberculosis significantly improves its detection in paucibacillary samples. 相似文献
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