中华哲水蚤线粒体DNA COI基因序列分析

采用苯酚-氯仿-异戊醇(25：24：1)提取、异丙醇沉淀、酒精洗涤、TE缓冲液保存方法提取了采自胶州湾青岛海域的中华哲水蚤基因组DNA；以相应引物经PCR扩增得到线粒体DNA细胞色素氧化酶Ⅰ亚基基因(mtCOD)片段；PCR产物采用化学法进行质粒重组(载体pGEM—T Easy Vector)、热休克转化重组质粒至大肠杆菌感受态细胞(JM109)、氨苄LB培养基扩大培养；测序．结果表明。中华哲水蚤线粒体COI碱基大小为710bp(国际基因库索引号AY665663)。其碱基组成A、C、G、T含量分别为24．23％、19．15％、22．54％和34．08％；G C含量为41．69％。A T为58．31％．     

裂足轮虫还是裂足臂尾轮虫

通过分析比较了裂足轮虫(Brachionus diuersicornis)、方型臂尾轮虫(B．quadridentatus)、壶状臂尾轮虫(B．urceus)、矩形臂尾轮虫(B．1eydigi)、萼花臂尾轮虫(B．calyciflorus)等5种轮虫的线粒体细胞色素氧化酶亚基I(COI）的部分序列，并结合4种海水臂尾轮虫的序列数据，用西氏晶囊轮虫(Asplanchna．sieboldi)作外群构建UPGMA树和NJ树，探讨了烈足轮虫的分类学问题，认为将裂足轮虫归属千臂尾轮虫属市为合适。     

Integrative taxonomy of Thalerosphyrus Eaton, 1881 (Ephemeroptera,Heptageniidae) in Thailand

ABSTRACT

A comprehensive revision of the genus Thalerosphyrus in Thailand is assessed. Four species are recognised: T. flowersi, T. sinuosus, T. vietnamensis and one new species, Thalerosphyrus thailandensis sp. nov., which is described here. A morphological comparison of the described species is provided. A Maximum Likelihood (ML) consensus tree based on 658 bp of the mitochondrial cytochrome oxidase I (COI) gene of five species was performed. Uncorrected p-distances strongly support the identity of these species, originally based on morphology, and show differences of 8.4–29.8% between species and 1.4–6.3% within species.

http://www.zoobank.org/urn:lsid:zoobank.org:pub:850FB31C-2B6A-4AB2-92D5-3413B193F196     

Description of the aberrant Leptopilina lasallei n. sp., with an updated phylogeny of Leptopilina Förster (Hymenoptera: Figitidae: Eucoilinae)

ABSTRACT

In the search for native Asian parasitoids of Drosophila suzukii, the notorious spotted-wing Drosophila (SWD), an odd new species of Eucoilinae was discovered. Leptopilina lasallei sp. nov. is herein described and diagnosed relative to other eucoilines associated with drosophilid hosts. Morphologically, L. lasallei is somewhat aberrant within Leptopilina; phylogenetically, L. lasallei is sister group to the core Leptopilina. In the process of investigating L. lasallei, a de novo molecular phylogeny of Leptopilina was generated and is included here. The integrated approach used for the characterisation of L. lasallei, and the resulting phylogeny of Leptopilina, produced data useful to select parasitoid species for SWD biological control.http://www.zoobank.org/urn:lsid:zoobank.org:act:402D504A-4616-4524-85D7-1C13A6276F06 http://www.zoobank.org/urn:lsid:zoobank.org:act:402D504A-4616-4524-85D7-1C13A6276F06     

Comparative genetic differentiation study of three coexisting mangrove crabs in western Atlantic

ABSTRACT

A multispecies approach may reveal the factors influencing the genetic differentiation of coexisting species along a wide geographic area. Our aim was to compare the genetic differentiation of mangrove crabs coexisting along the western Atlantic in order to verify if there are common barriers, using two mitochondrial DNA markers to quantify genetic differentiation, variance and diversity. In addition, we included a mismatch distribution to check demographic history. Our data revealed that species with similar pelagic larval duration had either high genetic differentiation or the absence of genetic structure. These results can be explained by factors that contribute to genetic differentiation, such as the presence of large estuarine areas (acting as barriers), ocean currents, and larval behaviour. Also, historical events that promoted the isolation of some areas in the past, such as glacial cycles during the Pleistocene, could have caused the observed high levels of genetic divergence in some species.     

中华按蚊的分子鉴定标准

中华按蚊（Anopheles sinensis）是重要的传疟媒介之一,广泛分布于中国和东南亚,它的形态特征和赫坎按蚊种团（Anopheles hyrcanus Group）其他蚊种十分近似,建立中华按蚊的分子鉴定标准十分重要。本研究从实验室中华按蚊品系的一个个体测序了线粒体DNA COI和COII基因,以及核糖体DNA D2、D3和ITS2位点,测序片段长度分别为658、663、551、365、556 bp。这些序列和近缘蚊种对应序列分别用最大似然法构建系统发育树,结果表明中华按蚊的序列均聚合为同一支。将测序的中华按蚊COII序列和已知蚊种的对应序列比对发现其中的保守位点分别是第133位点C,321位点C,382位点C,435位点T,516位点A和651位点C,线粒体DNA COI和COII基因序列的碱基组成具有AT偏向性,分别占69.15%和74.96%。提交了5条序列到NCBI数据库,其中核糖体DNA D2位点序列是首次在中华按蚊中公开报道。     

基于COI和D-Loop序列豫北虾虎鱼分子系统分析

虾虎鱼在河南省北部广泛分布,但其系统分类和多样性未有报道.从豫北原阳黄寺、安阳小南海、淇县淇河、长垣孙东闸、林州弓上水库等地采集虾虎鱼样本共计23尾,成都岷江样本6尾,分别扩增了线粒体COI基因和D-Loop序列.联合GenBank下载虾虎鱼的COI序列和D-Loop序列,构建了虾虎鱼的系统进化树.结果显示,采自黄寺、小南海、淇县、岷江地区的子陵吻虾虎鱼(Rhinogobius giurinus)聚为一个分支,而采自孙东闸和弓上水库的褐吻虾虎鱼(Rhinogobius brunneus)聚为一个单系群;这两种虾虎鱼遗传分化主要来自群体间.系统发育结果支持子陵吻虾虎鱼的韩国群体与中国群体构成姐妹群关系,中国不同地理种群间则相互交叉.     

厦门海域真刺唇角水蚤mtCOI序列分析

采用PCR扩增、克隆和序列测定等技术对厦门海域真刺唇角水蚤(Labidocera euchaetaGiesbrecht)线粒体细胞色素氧化酶I亚基基因(mitochondrial COI,mtCOI)进行了初步研究,得到3个雌性个体的mtCOI基因片段序列,序列的长度为709 bp,其A,T,C,G平均含量分别为23%、37%、15%和25%.三条核苷酸序列经比较后,得到其遗传相似性(i-dentity)为99.29%,共有15个差异位点,核苷酸差异均为颠换(transversion)类型.比较了厦门海域真刺唇角水蚤与GenBank数据库中的角水蚤科(Pontellidae)6个种的mtDNA COI基因片段序列(索引号AY145428、AY145429、AB206443、AB206444、AB206445、AB206446),其同源性为87.34%.文中讨论了上述7种桡足类的系统发生关系.     

Description of four new species of the Cirolana ‘parva group’ (Crustacea: Isopoda: Cirolanidae) from Thailand,with supporting molecular (COI) data

Four new species of the Cirolana ‘parva group’ from shallow coastal habitats in Thailand are described: Cirolana andamanensis sp. nov. and C. phangnga sp. nov., from the Andaman Sea, and C. siamensis sp. nov. and C. thailandica sp. nov. from the Gulf of Thailand. Molecular (The cytochrome oxidase subunit I gene) analysis incorporating taxa from the Cirolana ‘tuberculate group’ shows a monophyletic clade (Bayesian inference = 1 and maximum likelihood = 100%) within the Cirolana ‘parva group’ with two clades reflecting morphological characters. Cirolana andamanensis sp. nov. with antennula peduncle articles 1 and 2 entirely fused; male pereopod 1 without a setal fringe; uropod peduncle ventrolateral margin with 1 sensory seta. Cirolana phangnga sp. nov., C. siamensis sp. nov. and C. thailandica sp. nov. share the morphological characters of antennula peduncle articles 1 and 2 distinctly articulated; pereopod 1 in male with a setal fringe on the carpus and propodus; uropod peduncle ventrolateral margin with 3 sensory setae. Uncorrected p-distances strongly support the separation of these species, originally based on morphology, and show that the highest value of 32.1% is between C. thailandica sp. nov. and C. andamanensis sp. nov., while the lowest value of 22.5% is between C. phangnga sp. nov. and C. siamensis sp. nov.     

基于线粒体COI基因序列探讨长江华溪蟹的遗传分化

测定了长江华溪蟹3个亚种,包括指名亚种,桐柏亚种和陕县亚种共39个样本的线粒体细胞色素氧化酶亚基I（COI）基因部分的序列.重建的系统发生结果显示,长江华溪蟹分化成2个分支,一支代表雄性第1腹肢末节呈平直型的指名亚种支;另一支则代表雄性第1腹肢末节弯向背内方的内弯型支,包括稍向内弯的桐柏亚种和明显内弯的陕县亚种在内.在内弯型支内,桐柏亚种和陕县亚种之间的遗传距离为0.018,是两大分支间的1/10.提示长江华溪蟹COI基因的遗传分化与其雄性第1腹肢末节弯曲程度的形态分化基本一致.