APPLICATION OF HMB－45 MONOCLONAL ANTIBODY AND S100 PROTEIN IN THE IMMUNOHISTOCHEMICAL DIAGNOSIS OF MELANOMA

ＡＰＰＬＩＣＡＴＩＯＮＯＦＨＭＢ－４５ＭＯＮＯＣＬＯＮＡＬＡＮＴＩＢＯＤＹＡＮＤＳ１００ＰＲＯＴＥＩＮＩＮＴＨＥＩＭＭＵＮＯＨＩＳＴＯＣＨＥＭＩＣＡＬＤＩＡＧＮＯＳＩＳＯＦＭＥＬＡＮＯＭＡＬｉｕＤａｎｙａ（刘丹亚）；ＳｕＢａｏｓｈａｎ（苏宝山）；Ｘｕ...     

100%低地板现代有轨电车轻量化研究

100%低地板现代有轨电车具有节能、环保、投资小、载客量适中、乘坐舒适性高、后期维护费用低等特点,同时面临着严峻的轻量化及减重要求。对车体结构轻量化以及车体部件和内饰部件轻量化材料的应用开展研究,介绍了相关设计结构及应用方案。     

Investigation of tailpipe and evaporative emissions from China IV and Tier 2 passenger vehicles with different gasolines

The limited understanding of vehicular emissions in China, especially evaporative emissions, is one obstacle to establishing tighter standards. To evaluate tailpipe and evaporative emissions, two typical China IV vehicles and one Tier 2 vehicle with an onboard refuelling vapour recovery (ORVR) system were selected and tested. One of the China IV vehicles was fuelled with gasoline, E10 and M15, respectively, to investigate the effect of fuel properties on vehicular emissions. For each vehicle, cold-start tailpipe emission tests were conducted first, followed by an evaporation test. Based on the emission factors and real-world vehicle activity data, the annual tailpipe and evaporative hydrocarbon (HC) emissions of each vehicle were calculated and compared. The results show that E10 and M15 significantly reduced the tailpipe CO and particle number (PN) emissions but seriously aggravated the NOx emissions, especially for M15. The hot soak losses (HSLs) and diurnal breathing losses (DBLs) were slightly impacted by the fuel properties. The annual evaporative emissions with E10 and M15 were higher than that with gasoline. The ORVR system effectively controlled the evaporative emissions, especially for DBLs. Evaporative emissions from the China IV vehicles were 1.1–1.4 times the tailpipe HC emissions. Additionally, the evaporative emission factors of the China IV vehicles were almost 50% lower than the standard (2.0 g/test), whereas their annual evaporative emissions were almost 1.8–2.8 times higher than those from the Tier 2 vehicle. Therefore, controlling evaporative emissions currently remains a great need in China, and the ORVR might be a recommended evaporative control technology.     

姜黄素促进RAW264.7源性M1巨噬细胞向替代激活M2表型极化

目的观察姜黄素对LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)极化的影响及机制。方法不同浓度姜黄素(6.25、12.5、25μmol/L)干预LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)12h,同时再加入20μmol/L GW9662与25μmol/L姜黄素共同干预LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)12h,采用Real-time PCR、ELISA及Western blot方法检测IL-1β、IL-6和M2表型标志分子KLF4、FIZZ1、MGL1、PPARγ的表达,及阻断PPARγ后KLF4和FIZZ1的表达。结果不同浓度姜黄素均能上调LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)的M2标志分子的表达,并且抑制炎症因子IL-1β和IL-6的分泌;阻断PPARγ后,RAW264.7巨噬细胞源性M1表型巨噬细胞表达M2标志分子下调。结论姜黄素通过活化PPARγ促进LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)向M2表型极化。     

The Bid-rent Land Use Model of the simple,efficient, elegant,and effective model of land use and transportation

Integrated land use/transportation forecasting models add significant policy and infrastructure alternatives analysis capabilities to the urban planning process. The financial, time, and staff requirements to develop these models has put them beyond the reach of most small to medium sized urban areas. This paper presents the land use allocation submodel of the Simple, Efficient, Elegant, and Effective model of land use and transportation (SE³M), an integrated land use and transportation forecasting model founded upon Economic Base Theory and Bid-rent Theory. The Bid-rent Land Use Model (BLUM) is an agent based, spatial competition model utilizing unique utility curves for willingness to pay and incomes for budget constrained abilities to pay for each agent. The model structure, estimation, calibration, implementation, and validation are presented. With a single year of land use data available, the validation approach used the Kappa Index of Agreement to spatially check model outputs against base year control data while controlling for agreement by chance. The U.S. territory of Guam is used as the case study/proof of concept implementation for this model framework. Once calibrated, BLUM could solve the spatial competition problem on Guam in less than two minutes of processing time with over 90% accuracy.     

自卸式远洋散装水泥运输船特殊结构强度分析研究

以远洋散装水泥运输船为研究对象,采用三维有限元分析方法及ＤＮＶ船级社的ＳＥＳＡＭ结构计算软件,从船体结构强度、船舶振动预报和船体结构温度应力等方面对该型船结构设计的多项技术难点进行了详细的计算和评估。并且着重对大型散装水泥运输船间断内底板、中纵舱壁以及高腹板旁桁材等特殊结构的受力状态及应力分布进行分析和探讨。为自卸式散装水泥运输船的结构优化设计提供技术支撑。     

4MlE在施工项目管理中的拓展

通过对施工项目管理中"4M1E"的分析及其在当今项目管理中的拓展,为企业进行其项目管理提供理论依据.     

紧急疏散情况下地铁车站动态瓶颈识别

随着城市轨道交通系统逐渐成网,地铁车站日渐拥挤,为了制定科学有效的紧急疏散方案,有必要预先找到车站瓶颈点。基于时间可靠度函数模拟乘客在紧急情况下路径选择行为,在此基础上引入M//G/C/C排队模型对车站排队系统各个服务台进行排队性能指标计算,找出潜在瓶颈点。以广州地铁3号线岗顶站为例,结合车站硬件设施尺寸数量,模拟计算最恶劣情况下乘客疏散过程,找到3处潜在瓶颈点,测算结果与实际观测情况相符合,验证了该方法的有效性。     

基于施耐德M340PLC的船舶电站远程监视方法研究

实现了一种通过访问浏览器查看船舶电站中可编程逻辑控制器(PLC)数据的船舶电站监视方法。详细介绍了使用施耐德电气提供的Web Designer软件,开发"嵌入式Web服务器"上的网站,并将电站的运行数据发布到网站中的方法以及如何处理PLC中的运行数据,使其能够反映电站的实际运行情况。该方法在"上海海事大学-施耐德电气联合实验室"的船舶电站实验平台上得到实现。     

100 m定尺钢轨基地焊接500 m长钢轨施工技术

京津城际铁路设计采用100 m定尺钢轨基地焊接成500 m长钢轨,再用500 m长钢轨一次性铺设、焊接、锁定成跨区间无缝线路,引进Gaas80/580闪光接触焊轨机及配套的除锈、四向调直、精磨等成套设备,对100 m定尺钢轨焊接500 m长钢轨的焊轨生产线布局、焊轨流程及工序、质量控制要点等进行介绍.