Basic fibroblast growth factor and epidermal growth factor reverse impaired ulcer healing of the rabbit oral mucosa

BACKGROUND: The therapies for refractory ulcers on the oral mucosa are symptomatic and very unsatisfactory. We hypothesized that application of growth factors might be able to achieve successful remission of the lesion. We evaluated the effects of systemic administration and topical application of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on impaired wound healing of ulcers in the rabbit gingiva. METHODS: Almost uniform round ulcers could be created on the gingiva of the rabbits by chemical injury with acetic acid. When the submandibular glands were removed or i.v. injection of cisplatin (CDDP) and peplomycin sulfate was performed before ulcer formation, healing of the ulcers took longer than in untreated rabbits. To ascertain whether or not human EGF and bFGF affect rabbit cells, we first examined the effects of EGF and bFGF on the proliferation of the cells derived from rabbit gingiva. We then applied EGF or bFGF in these impaired healing models. RESULTS: EGF and bFGF promoted proliferation of the fibroblasts, and EGF also promoted proliferation of the keratinocytes isolated from gingival tissue of rabbits in vitro. Systemic injections of EGF and bFGF in rabbits, which had their submandibular glands removed, and topical application of bFGF accelerated healing of ulcers created in rabbits injected with CDDP and peplomycin sulfate. The ability of bFGF to promote the healing of ulcers was much greater than that of EGF. CONCLUSION: Basic FGF may be effective for refractory oral mucosal lesions.     

无血清培养人肝癌细胞增生率的变化和bFGF在细胞中的表达

采用无血清培养体系 ,研究肿瘤细胞自分泌调控机制。将人肝癌细胞培养在无血清无外源分裂因子培养基中 ,采用细胞原位计数法和免疫组织化学染色法 ,观察不同时相人肝癌细胞增生率的变化以及碱性成纤维细胞生长因子 (bFGF)的表达情况。结果 :人肝癌细胞生长良好 ,其增生速度与其分泌的bFGF量成正比。提示 :肝癌细胞在无外源生长因子的条件下通过分泌bFGF ,以自分泌和旁分泌的方式作用于自身和相邻细胞 ,促进细胞生长和增生。     

Sex cord-stromal tumors of the ovary

The developmental relationship between granulosa and stromal cells in the fetal ovary was reviewed. The proliferation and expression of basic fibroblast growth factor (bFGF) and luteinizing hormone receptor (LHR) of both the granulosa and theca cells was examined in the adult ovary. Ovarian granulosa cell tumors were then studied under light and electron microscopy and for immunohistochemical expression of bFGF and LHR. In the follicular development of the adult ovary, bFGF expression in the granulosa cells was correlated with theca cell proliferation. In ovarian granulosa cell tumors, estrogen-producing tumors showed a stratified or microfollicular pattern being accompanied by theca cells which had ultrastructural features of steroid-production, and these tumor cells exhibited immunoreactivity for bFGF. In contrast, tumors with no apparent hormone-production showed a solid or gyriform pattern without admixture of theca cells, and were negative for bFGF. LHR was expressed in both types of tumor. Accordingly, bFGF may play an important role in association with the theca cell component in granulosa cell tumors.     

MMP-2、TIMP-2、VEGF和bFGF在血管瘤增生及消退过程中的变化

目的 探讨基质金属蛋白酶-2（MMP-2）及其抑制剂（TIMP-2）、血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）在血管瘤增生、消退过程中的变化。方法 采用免疫组织化学链霉亲和素-过氧化酶（SP）法，检测增生期血管瘤32例、消退期血管瘤10例、血管畸形17例及小儿正常皮肤10例标本中MMP-2、TIMP-2、VEGF及bFGF的表达。结果 MMP-2、VEGF及bFGF在增生期血管瘤中的表达明显高于消退期血管瘤、血管畸形和正常皮肤，其差异有统计学意义；TIMP-2在消退期血管瘤中的表达明显高于增生期血管瘤、血管畸形和正常皮肤，其差异有统计学意义。结论 MMP-2、VEGF及bFGF可能促进血管瘤血管生成，从而促进血管瘤增生，而TIMP-2可能促进血管瘤消退。     

PTTG-bFGF通路在人脑星形细胞肿瘤中的表达及相关性研究

目的 探讨垂体瘤转化基因(PTTG)和碱性成纤维细胞生长因子(bFGF)在人脑星形细胞肿瘤中的表达及其意义.方法 免疫组化SP法检测55例人脑星形细胞肿瘤中PTTG、bFGF的表达.结果 各级别肿瘤中均不同程度发现PTTG、bFGF阳性表达,二者表达强度有随肿瘤恶性程度增高而增强的趋势(rS分别为0.8757、0.7557,P＜0.01),PTTG、bFGF的表达呈正相关(rS=0.7520,P＜0.01).结论 PTTG-bFGF通路存在于人脑星形细胞肿瘤中,与肿瘤恶性程度密切相关,二者在胶质瘤的发生及恶性转化中具有重要作用.     

Ad-METH1对培养的内皮细胞分泌活性的影响

目的：研究基因重组血管抑制剂Ad-METH1（metalloprotease and thrombospondin1）对培养的内皮细胞分泌活性的影响,探讨血管生成抑制防治增生性瘢痕的可能机制。方法：制备重组血管抑制剂Ad-METH1,转染培养的人脐静脉内皮细胞,通过放射免疫分析法及酶联免疫分析法研究Ad-METH1对内皮细胞分泌内皮素-1（endothelin-1,ET-1）及碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）的影响。结果：基因转染后24h内皮细胞ET-1、bFGF分泌受到明显抑制。结论：Ad-METH1对内皮细胞ET-1、bFGF分泌活性有影响,此是其早期参与抑制增生性瘢痕形成的可能机制之一。     

Time-dependent changes in distribution of basic fibroblast growth factor immunoreactive cells in hippocampus after kainic acid injection in rat pups

Five-day-old Wistar albino rats were injected with kainic acid (KA) or saline i.p. to investigate time-dependent alterations in morphology and number of basic fibroblast growth factor (bFGF) immunoreactive (-ir) astrocytes and neurons in hippocampus at 15, 30, and 90 days after the injections. Sections were stained with cresyl violet for morphological evaluation and bFGF immunohistochemistry was used for quantitative evaluation of bFGF-ir cell density. Fifteen days after KA injection, there was gliosis but no neuronal loss although disorganization in CA1, CA3, CA4 pyramidal layers and neuronal loss were evident 30 and 90 days after the injection. KA injected rats demonstrated significantly increased number of bFGF-ir astrocytes throughout the hippocampus and pyramidal neurons in CA2 after 15 days and decreased number of bFGF-ir cells after 30 and 90 days. The decrease in the number of bFGF-ir astroglia and neurons in long term after KA injection may indicate a decrease in the production of bFGF and/or number of bFGF-ir cells suggesting that protective effects of bFGF may be altered during epileptogenesis in hippocampus.     

转化生长因子-β和碱性成纤维细胞因子在儿童原发性局灶节段性肾小球硬化肾组织中的表达

目的：通过检测转化生长因子-β（TGF-β）和碱性成纤维细胞生长因子（bFGF）在儿童原发性局灶节段性肾小球硬化（FSGS）肾组织中的表达情况,并分析其与肾小管间质病理变化的关系,以了解TGF-β与bFGF在原发性FSGS发生发展中的作用。方法：选择肾活检明确诊断为原发性FSGS患儿的肾组织共43例,其中不伴有肾小管间质病变的FSGS肾组织共16例,设为实验1组;伴有肾小管间质病变的FSGS肾组织共27例,为实验2组。另将同期因孤立性血尿入院肾活检证实为非FSGS、病理改变较轻的肾组织作为对照组,共17例。采用免疫组化法检测细胞/生长因子TGF-β、bFGF在各组中的表达。通过方差分析（ANOVA）和相关分析法分析细胞/生长因子的表达与FSGS肾组织病理变化的关系以及细胞/生长因子之间的相互作用关系。结果：TGF-β、bFGF在各组肾组织中均有表达,表达量在对照组、实验1组和实验2组中依次升高,各组间的差异均具有统计学意义（P〈0.05）;且TGF-β和bFGF的表达与肾小管间质指数呈正相关,相关系数依次为0.763和0.661。此外,TGF-β和bFGF两者的表达量经相关分析也显示呈正相关,相关系数为0.587。结论：TGF-β和bFGF在原发性FSGS患儿肾组织中高表达;随着FSGS的发展,它们在肾组织中表达量不断增加,促使肾小管间质向纤维化发展,而且两者在促进肾脏纤维化具有一定的协同作用。     

慢性低氧性肺动脉高压大鼠血清碱性成纤维细胞生长因子含量的变化

为探讨碱性成纤维细胞生长因子（ｂＦＧＦ）在慢性低氧性肺动脉高压肺血管重建中的作用及机理，采用慢性低氧性肺动脉高压大鼠模型，用酶联免疫吸附法测定其血清ｂＦＧＦ含量，并用原位杂交法观察肺、心、脑、肾等器官ｂＦＧＦｍＲＮＡ表达的变化。结果显示：低氧组血清ｂＦＧＦ含量（３５．９±２３．５ｐｇ．ｍｌ－１）明显高于对照组（６．３０±０．９７ｐｇ．ｍｌ－１，Ｐ＜０．００５）；低氧组肺小动脉ｂＦＧＦｍＲＮＡ表达明显增强，而心、脑、肾ｂＦＧＦｍＲＮＡ表达无变化。提示ｂＦＧＦ参与了慢性低氧性肺动脉高压肺小动脉重建的调控。     

人表皮生长因子与碱性成纤维细胞生长因子纳米缓释剂对成纤维细胞的影响

目的:制备碱性成纤维细胞生长因子(bFGF)、人表皮生长因子(EGF)可降解缓释微球,考察其生物活性的保存情况,以及它们对成纤维细胞的作用。方法:采用改良的乳化冷凝法交联制备复合bFGF、EGF的明胶缓释微球,将它们加入成纤维细胞的培养液中,用细胞计数法、四甲基偶氮唑盐微量反应比色法(MTT法)测定细胞增殖情况。结果:复合bFGF、EGF的缓释微球平均粒径(11.32±3.64)μm;培养1天后各组细胞计数、吸光度(A)值差异均无显著性;5天后,两种生长因子缓释微球组细胞计数、吸光度(A)值明显高于对照组;7天后,两种生长因子缓释微球组值仍高于其它组,但差异无显著性。结论:复合bFGF、EGF的缓释微球制备工艺简便,成球性好;能较长时间地持续释放活性bFGF、EGF,可促进成纤维细胞的增殖。