嵌入式Web控制终端的物联网移动E-Laboratory平台

为了进一步提高大学校园设备资源的利用率,实现多种实验装置的网络化控制和用户终端移动化,基于物联网技术,将LAMP移植到采用ARM架构的微处理器上,通过搭建嵌入式Web控制终端,对Web服务平台进行了详细的设计,实现了多种实验装置的网络化控制;将单自由度机械臂系统这一实验装置与EWC_T连接,基于Android操作系统上开发了一个应用,设计并搭建了Mobile Experiment系统,实现了网络化实验室平台的用户终端的移动化;通过实验证明该系统能够可靠地完成对实验装置的远程控制,并具有低功耗、低成本和便携性好等优点。     

基于LAMP的高性能Web服务器的架构

文章提出了基于LAMP的高性能Web服务器的架构方案,采用了Apache日志、Webalizer日志分析、Cacti流量监控、入侵检测的方法,架构了一个完善的、稳定的、安全的、低廉的高性能Web服务器,满足了中小型企业的要求。     

Compact optical diagnostic device for isothermal nucleic acids amplification

We recently reported the successful use of the loop-mediated isothermal amplification (LAMP) reaction for hepatitis B virus (HBV) DNA amplification and its optimal primer design method. In this study, we report the development of an integrated isothermal device for both amplification and detection of targeted HBV DNA. It has two major components, a disposable polymethyl methacrylate (PMMA) micro-reactor and a temperature-regulated optical detection unit (base apparatus) for real-time monitoring of the turbidity changes due to the precipitation of DNA amplification by-product, magnesium pyrophosphate. We have established a correlation curve (R² = 0.99) between the concentration of pyrophosphate ions and the level of turbidity by using a simulated chemical reaction to evaluate the characteristics of our device. For the applications of rapid pathogens detection, we also have established a standard curve (R² = 0.96) by using LAMP reaction with a standard template in our device. Moreover, we also have successfully used the device on seven clinical serum specimens where HBV DNA levels have been confirmed by real-time PCR. The result indicates that different amounts of HBV DNA can be successfully detected by using this device within 1 h.     

在Ubuntu平台上部署基于Snort的入侵检测系统

Snort是一套免费的轻量级入侵检测系统,当今的很多入侵检测系统都以其为参照。虽然Snort软件体积非常小巧,但是其部署有一定的难度。为了以更直观的方式监测系统的运行状况,通常部署Snort系统时除了要安装Snort软件本身外,还需要大量的相关软件支持。文章介绍了在Ubuntu平台上架设snort系统的具体过程。系统以MysQL数据库存储入侵检测信息,用BASE工具显示检测结果。     

基于开源LAMP架构的Web打印技术

通过对现有B/S架构打印方法进行分析对比,针对它们的缺陷提出了一种基于开源LAMP架构的跨平台、精确、有效的Web打印新技术,并详细介绍了其原理,最后给出了具体的实现方法.     

Real time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus

We evaluated the usefulness of the loop-mediated isothermal amplification (LAMP) using a portable ESE Quant tube scanner as a rapid and simple method for the detection of Vibrio parahaemolyticus, an important pathogen causing seafood-borne gastroenteritis. The real time LAMP (RT-LAMP) assay using a hemolysin gene (tlh/ldh)-specific primers was verified using V. parahaemolyticus strains (n = 91) from different countries and other non-target strains to check the utility of this method. Both the sensitivity and specificity of the RT-LAMP using 3 pairs of tlh/ldh-specific primers developed in this study were excellent (100%). The detection limit of the RT-LAMP was as low as 7 Colony forming unit per reaction and detection time was only 20 min. Comparative evaluation of the target bacterial strains with the RT-LAMP using ESE Quant tube scanner, API 20E system and conventional RT-PCR method revealed that the RT-LAMP assay developed in this study is simpler and more rapid than the latter two methods. Therefore, the RT-LAMP method using the easily portable ESE Quant tube scanner can be considered as an effective tool for the rapid screening of V. parahaemolyticus strains in environmental and clinical samples, especially, in remote areas of developing countries during epidemic periods.     

Effect of Penicillium nordicum contamination rates on ochratoxin A accumulation in dry-cured salami

Fungal starter, such as Penicillium nalgiovense, are commonly used to inoculate sausages before seasoning process. However, Penicillium nordicum, a well-known ochratoxin A (OTA) producer frequently isolated from seasoning rooms, could colonize the casing surface during the early stage of production. The relationship between OTA accumulation and simultaneous inoculation of P. nalgiovense and P. nordicum at different rates was evaluated. After 14 days of seasoning, the persistence of P. nordicum was assessed by LAMP assay revealing its capability to colonize and grow on salami surface at all the contamination rates. At the end of seasoning, OTA was accumulated both in mycelium and dry-cured meat when P. nordicum contamination rate ranged from 25% to 100% of inoculum, while no OTA was detected in dry-cured meat at 2.5% and 0.25%. Results demonstrated that contamination of fungal starter by P. nordicum could represent a serious concern during salami production and therefore represents an important critical point to be monitored.     

快速检测淋球菌porA和16S rRNA基因的环介导等温扩增技术的建立

目的建立快速检测淋球菌porA和16S rRNA基因的环介导等温扩增(Loop-mediated isothermal amplification,LAMP)技术,探讨其用于淋球菌感染早期诊断的可行性。方法利用Primer-ExplorerV3软件分别针对淋球菌porA及16S rRNA基因设计6条引物(2条内引物FIP、BIP,2条外引物F3、B3,2条环引物LF、LB),以淋球菌标准菌株基因组DNA为模板,进行LAMP扩增,同时,以外引物F3、B3为PCR引物,进行PCR扩增,并比较2种扩增方法的灵敏度和特异性。结果LAMP法与PCR法灵敏度相同,可检测到10个拷贝的目的基因;其针对淋球菌porA和16S rRNA基因的引物对脑膜炎奈瑟菌、肺炎链球菌和大肠埃希菌的DNA均不能扩增。结论已成功建立了检测淋球菌porA和16S rRNA基因的LAMP技术,为淋球菌的快速检测提供了新的手段,有望成为淋球菌常规检测的简便方法。     

环介导等温核酸扩增技术快速检测产毒亚历山大藻

DNA环介导等温扩增(LAMP)方法是一种新型的核酸扩增技术,该方法是在等温的条件下进行的,具有反应时间短、灵敏度高、特异性强的特点.本文以产麻痹性贝毒(PSP)的亚历山大藻为研究对象,采用简易法提取DNA模板,设计特异性LAMP引物,利用LAMP技术进行产毒藻种的快速检测,同时,着重对LAMP技术与PCR技术在检测微小亚历山大藻细胞的灵敏度方面做了比较.向LAMP终产物中加入SYBR Green I 染料后可直接用肉眼观察结果而不需要通过凝胶电泳来观察.结果表明,LAMP技术在恒温65℃,1h内就可以检测到产毒藻种;LAMP技术检测微小亚历山大藻的最低检测限为200个/ml,而PCR技术的最低检测限为1000个/ml,LAMP扩增方法比PCR扩增方法的程序简单、反应时间短、灵敏度高;LAMP技术不需要精密的温度循环装置,有恒温加热设备就可以满足检测条件,可用于野外检测.     

职业规划应用平台运行环境环境分析

LAMP环境主要是指在Linux操作系统上使用Apache、Mysql、PHP软件搭建的动态网站和开源服务器环境。研究在Linux发行版本Cent OS上实现网站环境的优化。Cent OS环境下面搭建的网站环境相对windows环境具有很大优势,但采用新的技术手段对提高环境的访问速度、处理能力、安全性是很有必要的,对降低硬件成本有相当大帮助。